RSS-Feed abonnieren
DOI: 10.1055/s-0038-1665445
Up-regulation of Tissue Factor Expression by Platelet-derived Growth Factor in Human Vascular Smooth Muscle Cells in Culture
Role of Mitogen-Activated Protein Kinase and Effects of Intracellular Cyclic AMPPublikationsverlauf
Received 02. 1997
Accepted 16. Juli 1997
Publikationsdatum:
12. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199701/lookinside/thumbnails/10-1055-s-0038-1665445-1.jpg)
Summary
Human vascular smooth muscle cells (SMCs) isolated from normal adult arteries were investigated for the expression of Tissue Factor (TF) procoagulant activity at their membrane surface and TF antigen in lysed cells. In growing conditions, SMCs expressed high levels of TF activity and antigen. When cells were made quiescent by 72 h of subculture in serum-poor medium, these levels fell to about one third of the initial values. Platelet-derived growth factor BB (PDGF) up-regulated in a dose-dependent manner TF expression with a significant increase (1.8 fold) within five hours. PD 98059, a specific inhibitor of mitogen-activated protein kinase (MAPK) pathway involved in cell response to PDGF, also prevented TF up-regulation. Short-term treatment by Pentoxifylline and dibutyryl cAMP also completely prevented induced TF up-regulation, but remained without effect on baseline levels of quiescent, unstimulated cells. At their effective concentrations, pentoxifylline and dibutyryl cAMP also inhibited the activation of MAPK induced by PDGF. The rapid induction of TF upon growth factor stimulation may be important in circumstances where SMCs proliferate, such as vascular events or vessel development.
-
References
- 1 Zur M, Nemerson Y. Tissue Factor pathways of blood coagulation. Haemostasis and Thrombosis. Bloom AL, Thomas DP. (Eds) Churchill Livingston; – Edinburgh: 1987: 148-163
- 2 Toschi V, Gallo R, Lettino M, Fallon JT, Gertz SD, Fernández-Ortiz A, Chesebro JH, Badimon L, Nemerson Y, Fuster V, Badimon JJ. Tissue Factor modulates the thrombogenicity of human atherosclerotic plaques. Circulation 1997; 95: 594-599
- 3 Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Am J Pathol 1989; 134: 1087-1097
- 4 Mackman N. Regulation of the tissue factor gene. FASEB J 1995; 9: 883-889
- 5 Sanders M. Molecular and cellular concepts in atherosclerosis. Pharmac Ther 1994; 61: 109-153
- 6 Wilcox JN, Smith KM, Schwartz SM, Gordon D. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque. Proc Natl Acad Sci USA 1989; 86: 2839-2843
- 7 Thiruvikraman SV, Guha A, Roboz J, Taubman MB, Nemerson Y, Fallon JT. In situ localization of tissue factor in human artherosclerotic plaques by binding of digoxigenin-labeled factors VIIa and X. Lab Invest 1996; 75: 451-461
- 8 Marmur JD, Rossikhina M, Guha A, Fyfe B, Friedrich V, Mendlowitz M, Nemerson Y, Taubman MB. Tissue factor is rapidly induced in arterial smooth muscle after balloon injury. J Clin Invest 1993; 91: 2253-2259
- 9 Camerer E, Kolsto AB, Prydz H. Cell biology of tissue factor the principal initiator of blood coagulation. Thromb Res 1996; 81: 01-41
- 10 Maynard JR, Dreyer BE, Stemerman MB, Pitlick FA. Tissue-factor coagulant activity of cultured human endothelial and smooth muscle cells and fibroblasts. Blood 1977; 50: 387-396
- 11 Taubman MB, Marmur JD, Rosenfield CL, Guha A, Nichtberger S, Nemerson Y. Agonist-mediated tissue factor expression in cultured vascular smooth muscle cells. Role of Ca2+ mobilization and protein kinase C activation. J Clin Invest 1993; 91: 547-552
- 12 Catalioto RM, Cucchi P, Renzetti AR, Criscuoli M, Subissi A. Human umbilical vein smooth muscle cells as a model to study thrombin generation and function: effect of thrombin inhibitors. Thromb Haemost 1996; 76: 603-609
- 13 Tremoli E, Prati L, Parolari A, Antona C, Colli S, Mussoni L. Serum and thrombin induce tissue factor expression in human smooth muscle cells. Thromb Haemost (Abstract) 1995; 73: 1080
- 14 Glowes AW, Glowes MM. Kinetics of cellular proliferation after arterial injury. II. Inhibition of smooth muscle growth by heparin. Lab Invest 1985; 52: 611-616
- 15 Dartsch PC, Bauriedel G, Schimko I, Weiss HD, Hofling B, Betz E. Cell constitution and characterization of human atherosclerotic plaque selectivity removed by percutaneous atherectomy. Atherosclerosis 1989; 80: 149-157
- 16 Dartsch PC, Voisard R, Bauriedel G, Höfling B, Betz E. Growth characteristics and cytoskeletal organization of cultured smooth muscle cells from primary stenosing and restenosing lesions. Arteriosclerosis 1990; 10: 62-75
- 17 Bomfeldt KE. Intracellular signaling in arterial smooth muscle migration versus proliferation. Trends Cardiovasc Med 1996; 6: 143-151
- 18 Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362: 801-809
- 19 Archipoff G, Beretz A, Brisson C. Effect of pentoxifylline on the modulation by cytokines of thrombomodulin and tissue factor activites on the surface of human endothelial cells in culture. In: Pentoxifylline and analogues: effects on leukocyte function. Hakim J, Mandell GL, Novicl Jr WJ. eds Basel; Karger: 1990: 48-52
- 20 Ollivier V, Ternisien C, Vu T, Hakim J, De Prost D. Pentoxifylline inhibits the expression of tissue factor mRNA in endotoxin-activated human monocytes. FEBS Lett 1993; 322: 231-234
- 21 Ollivier V, Houssaye S, Temisien C, Léon A, De Vemeuil H, Elbim C, Mackman N, Edgington TS, De Prost D. Endotoxin-induced tissue factor messenger RNA in human monocytes is negatively regulated by a cyclic AMP-dependent mechanism. Blood 1993; 81: 973-979
- 22 Leclerc NE, Haan-Archipoff G, Lenoble M, Beretz A. Inhibitors of phosphodiesterase (Pentoxifylline, Trequinsin) inhibit apical and subcellular matrix expression of tissue factor in cultured human endothelial cells. J Cardiovasc Pharmacol 1995; 25: 88-91
- 23 Cobb MH, Goldsmith EJ. How MAP Kinases are regulated. J Biol Chem 1995; 270: 14843-14846
- 24 Pinzani M, Marra F, Caligiuri A, DeFranco R, Gentilini A, Failli P, Gentilini P. Inhibition by pentoxifylline of extracellular signal-regulated kinase activation by platelet-derived growth factor in hepatic stellate cells. Brit J Pharmacol 1996; 119: 1117-1124
- 25 Fallier-Becker P, Rupp J, Fingerle J, Betz E. Smooth muscle cells from rabbit aorta. In: Cell culture techniques in Heart and Vessel Research. Piper HH. (ed.) Springer-Verlag-Heidelberg; 1990: 247-269
- 26 Chamley-Campbell J, Campbell GR, Ross R. The smooth muscle cell in culture. Physiol Reviews 1979; 59: 01-61
- 27 Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G. A monoclonal antibody againstα-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 1986; 103: 2787-2796
- 28 Osborn M, Weber K. Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures. Methods in cell biology 1982; 24: 097-132
- 29 Vindelov L, Christensen J. A review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. Cytometry 1990; 11: 753-770
- 30 Magnier-Gaubil C, Herbert JM, Quarck R, Papp B, Corvazier E, Wuytack F, Lévy-Tolédano S, Enouf J. Smooth muscle cell cycle and proliferation. J Biol Chem 1996; 271: 27788-27794
- 31 Breton M, Berrou E, Brahimi-Horn MC, Deudon E, Picard J. Synthesis of sulfated proteoglycans throughout the cell cycle in smooth muscle cells from pig aorta. Exper Cell Res 1986; 166: 416-426
- 32 Diquélou A, Dupouy D, Gaspin D, Constans J, Sié P, Boneu B, Sakariassen KS, Cadroy Y. Relationship between endothelial tissue factor and thrombogenesis under blood flow conditions. Thromb Haemost 1995; 74: 778-783
- 33 Drysdale BE, Zacharchuk CM, Shin HS. Mechanism of macrophage-mediated cytotoxicity: production of a soluble cytotoxic factor. J Immunol 1993; 131: 2362-2367
- 34 Cadroy Y, Gaspin D, Dupouy D, Lormeau JC, Boneu B, Sié P. Heparin reverses the procoagulant properties of stimulated endothelial cells. Thromb Haemost 1996; 75: 190-195
- 35 Hakim J. Pharmacologic control of intracellular signaling pathways: from research to therapy. Cardiovasc Pharmacol 1995; 25: 106-113
- 36 Wu J, Dent P, Jelinek T, Wolfman A, Weber MJ, Sturgill TW. Inhibition of the EGF activated MAP kinase signaling pathway by adenosine 3’5’ monophosphate. Science 1993; 262: 1065-1069
- 37 Cook SJ, McCormick F. Inhibition by cAMP of ras-dependent activation of Raf. Science 1993; 262: 1069-1072
- 38 Bouchard BA, Shatos MA, Tracy PB. Human brain pericytes differentially regulate expression of procoagulant enzyme complexes comprising the extrinsic pathway of blood coagulation. Arterioscler Thromb Vase Biol 1997; 17: 1-9
- 39 Busso N, Huet S, Nicodeme E, Hiemaux J, Hyafil F. Refractory period phenomenon in the induction of tissue factor expression on endothelial cells. Blood 1991; 78: 2027-2035
- 40 Gramzinski RA, Broze GJ, Carson SD. Human fibroblast tissue factor is inhibited by lipoprotein associated coagulation inhibitor and placental anticoagulant protein but not by apolipoprotein A-II. Blood 1989; 73: 983-989
- 41 Hartzell S, Ryder K, Lanahan A, Lau LF, Nathans D. A growth factor-responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to human tissue factor. Mol and Cell Biol 1989; 9: 2567-2573
- 42 Verhagen HJ, Heijnen-Snyder GJ, Vink T, Pronk A, Van Vroonhoven TJ, Eikelboom BC, Sixma JJ, De Groot PG. Tissue factor expression on mesothelial cells is induced during in vitro culture – Manipulation of culture conditions creates perspectives for mesothelial cells as a source for cell seeding procedures on vascular grafts. Thromb Haemost 1995; 74: 1096-1102
- 43 Pledger WJ, Hart CA, Locatell KL, Scher CD. Platelet-derived growth factor-modulated proteins: Constitutive synthesis by a transformed cell line. Proc Natl Acad Sci USA 1981; 78: 4358-4362
- 44 Servant MJ, Giasson E, Meloche S. Inhibition of growth factor-induced protein synthesis by a selective MEK inhibitor in aortic smooth muscle cells. J Biol Chem 1996; 271: 16047-16052
- 45 Dudley DT, Pang L, Decker SJ, Bridges AJ, Saltiel AR. A synthetic inhibitor of the mitogen-activated protein kinase cascade. Proc Natl Acad Sci USA 1995; 92: 7686-7689
- 46 Crutchley DJ, Hirsh MJ. The stable prostacyclin analogiloprost, and prostaglandin E1 inhibit monocyte procoagulant activity in vitro. Blood 1991; 78: 382-386
- 47 Hasday JD, Sitrin RG. Dipyridamole stimulates urokinase production and suppresses procoagulant activity of rabbit alveolar macrophages: a possible mechanism of antithrombotic action. Blood 1987; 69: 660-667
- 48 Malarkey K, Belham CM, Paul A, Graham A, McLees A, Scott PH, Plevin R. The regulation of tyrosine kinase signalling pathways by growth factor and G-protein-coupled receptors. Biochem J 1995; 309: 361-375
- 49 Peterson T. Pentoxifylline prevents fibrosis in an animal model and inhibits platelet-derived growth factor-driven proliferation of fibroblasts. Hepatology 1993; 17: 486-493
- 50 Mackman N, Brand K, Edgington TS. Liposaccharide mediated transcroptional activation of the human tissue factor gene in THP-1 monocytic cells require both activator protein 1 and nuclear factor κB binding site. J Exp Med 1991; 174: 1517-1526
- 51 Parry GCN, Mackman N. Transcriptional regulation of tissue factor expression in human endothelial cells. Arterioscler Thromb Vasc Biol 1995; 15: 612-621
- 52 Ollivier V, Parry GCN, Cobb R, de Prost D, Mackman N. Elevated cyclic AMP inhibits NF-κB mediated transcription in human monocytic cells and endothelial cells. J Biol Chem 1996; 271: 20828-20835
- 53 Taby O, Rosenfield CL, Bogdanov V, Nemerson Y, Taubman MB. Cloning of the rat tissue factor DNA and promoter: identification of a serum response region. Thromb Haemost 1996; 76: 697-702
- 54 Carmeliet P, Mackman N, Moons L, Luther T, Gressens P, Van Vlaenderen I Demunck, Hasper M, Breier G, Evrard P, Müller M, Risau W, Edgington T, Collen D. Role of tissue factor in embryonic blood vessel development. Nature 1996; 383: 73-75
- 55 Tommey JR, Kratzer KE, Lasky NM, Stanton JJ, Broze GJ. Targeted disruption of the murine tissue factor gene results in embryonic lethality. Blood 1996; 88: 1583-1587