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Thromb Haemost 1986; 56(03): 343-348
DOI: 10.1055/s-0038-1666778
Original Article
Schattauer GmbH Stuttgart

Immunoradiometric Assays for Human Coagulation Factor VII Using Polyclonal Antibodies Against the Ca(II)-Dependent and Ca(II)-Independent Conformation

V J J Bom
The Haemostasis and Thrombosis Research Unit, Leiden University Hospital, Leiden, The Netherlands
,
N H van Tilburg
The Haemostasis and Thrombosis Research Unit, Leiden University Hospital, Leiden, The Netherlands
,
C Krommenhoek-van Es
The Haemostasis and Thrombosis Research Unit, Leiden University Hospital, Leiden, The Netherlands
,
R M Bertina
The Haemostasis and Thrombosis Research Unit, Leiden University Hospital, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 02 June 1986

Accepted after revision 11 September 1986

Publication Date:
18 July 2018 (online)

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Summary

Human coagulation factor VII is a trace plasma protein belonging to the vitamin K-dependent factors. Two specific and sensitive immunoradiometric assays for factor VII were developed using immunopurified rabbit antibodies against the Ca(II)-independent and Ca(II)-dependent conformation of factor VII. Both assays were insensitive to the activation state of factor VII. The distribution of factor VII antigen was studied in 40 healthy individuals and the antigen level in normal plasma was calculated to be 0.52-0.62 μg/ml. The two assays were used in a comparative study of factor VII procoagulant activity and factor VII antigen in patients treated with oral anticoagulants.

Key words

Factor VII - Immunoradiometric assay - Calcium - Oral anticoagulation - Factor VII deficiency
 

  • References

  • 1 Østerud B. Activation pathways of the coagulation system in normal haemostasis. Scand J Haematol 1984; 32: 337-345
    PubMedGoogle Scholar
  • 2 Østerud B, Rapaport SI. Activation of factor IX by the reaction product of tissue factor and factor VII: additional pathway for initiating blood coagulation. Proc Natl Acad Sci USA 1977; 74: 5260-5264
    CrossrefPubMedGoogle Scholar
  • 3 Morrison SA, Jesty J. Tissue factor-dependent activation of tritium-labeled factor IX and factor X in human plasma. Blood 1984; 63: 1338-1347
    PubMedGoogle Scholar
  • 4 Broze Jr GJ, Majerus PW. Purification and properties of human coagulation factor VII. J Biol Chem 1980; 255: 1242-1247
    PubMedGoogle Scholar
  • 5 Bajaj SP, Rapaport SI, Brown SF. Isolation and characterization of human factor VII. Activation of factor VII by factor Xa. J Biol Chem 1981; 256: 253-259
    PubMedGoogle Scholar
  • 6 Masys DR, Bajaj SP, Rapaport SI. Activation of human factor VII by activated factors IX and X. Blood 1982; 60: 1143-1150
    PubMedGoogle Scholar
  • 7 Seligsohn U, Østerud B, Brown SF, Griffin JH. Activation of human factor VII in plasma and in purified systems. Roles of activated factor IX, kallikrein and activated factor XII. J Clin Invest 1979; 64: 1056-1065
    PubMedGoogle Scholar
  • 8 Laake K, Østerud B. Activation of purified plasma factor VII by human plasmin, plasma kallikrein and activated components of the human intrinsic blood coagulation system. Thromb Res 1974; 5: 759-772
    CrossrefPubMedGoogle Scholar
  • 9 Broze Jr GJ, Majerus PW. Isolation and properties of human factor VII. Clin Res 1979; 27: 459A
    PubMedGoogle Scholar
  • 10 Dam-Mieras MCE van, Hemker HC. Half-life time and control frequency of vitamin K-dependent coagulation factors. Theoretical considerations on the place of factor VII in the control of oral anticoagulant therapy. Haemostasis 1983; 13: 201-208
    PubMedGoogle Scholar
  • 11 Mariani G, Mazzucconi MG, Solinas S, Avvisati G, Chistolini A, Morretti T. Studies on PIVKA VII. Haemostasis 1984; 14: 238-243
    PubMedGoogle Scholar
  • 12 Kisiel W, McMullen BA. Isolation and characterization of human factor Vila. Thromb Res 1981; 22: 375-380
    CrossrefPubMedGoogle Scholar
  • 13 Deijk WA van, Dam-Mieras MCE van, Muller AD, Hemker HC. Evaluation of a coagulation assay determining the activity state of factor VII in plasma. Haemostasis 1983; 13: 192-197
    PubMedGoogle Scholar
  • 14 Seligsohn U, Østerud B, Rapaport SI. Coupled amidolytic assay for factor VII: its use with a clotting assay to determine the activity state of factor VII. Blood 1978; 52: 978-988
    PubMedGoogle Scholar
  • 15 Goodnight Jr SH, Feinstein DI, Østerud B, Rapaport SI. Factor VII antibody neutralizing material in hereditary and acquired factor VII deficiency. Blood 1971; 38: 1-8
    PubMedGoogle Scholar
  • 16 Denson K WE. The levels of factors II, VII, IX and X by antibody neutralisation techniques in the plasma of patients receiving phenan-dione therapy. Br J Haematol 1971; 20: 643-648
    CrossrefPubMedGoogle Scholar
  • 17 Briët E, Loeliger EA, Tilburg NH van, Veltkamp JJ. Molecular variant of factor VII. Thromb Haemostas 1976; 35: 289-294
    PubMedGoogle Scholar
  • 18 Fair DS. Quantitation of factor VII in the plasma of normal and warfarin-treated individuals by radioimmunoassay. Blood 1983; 62: 784-791
    PubMedGoogle Scholar
  • 19 Mikami S, Tuddenham E GD. Studies on immunological assay of vitamin K-dependent factors. I. Measurement of factor VII antigen by radioimmunoassay. Br J Haematol 1986; 62: 171-181
    PubMedGoogle Scholar
  • 20 Broze Jr GJ, Hickman S, Miletich JP. Monoclonal anti-human factor VII antibodies. Detection in plasma of a second protein antigenically and genetically related to factor VII. J Clin Invest 1985; 76: 937-946
    CrossrefPubMedGoogle Scholar
  • 21 Loeliger EA, Lewis SM. Progress in laboratory control of oral anticoagulants. Lancet 1982; 2: 318-320
    PubMedGoogle Scholar
  • 22 Bertina RM, Orlando M, Tiedemann-Alderkamp G HJ. Preparation of a human factor VII deficient plasma. Thromb Res 1978; 13: 537-541
    CrossrefPubMedGoogle Scholar
  • 23 Bom V JJ, Ram IE, Alderkamp G HJ, Reinalda-Poot JH, Bertina RM. Application of factor VH-Sepharose affinity chromatography in the purification of human tissue factor apoprotein. Thromb Res 1986; 42: 635-643
    CrossrefPubMedGoogle Scholar
  • 24 Broze Jr GJ, Majerus PW. Human factor VII. Meth Enzymol 1980; 80: 228-237
    PubMedGoogle Scholar
  • 25 Tankersley DL, Alving BM, Finlayson JS. Preparation of β-XIIa (Hageman factor fragment) from human plasma. Thromb Res 1982; 25: 307-317
    CrossrefPubMedGoogle Scholar
  • 26 Fraker PJ, Speck Jr JC. Protein and cell membrane iodonations with a sparingly soluble chloramide, 1,3,4,6-tetrachloro-3a,6a-diphenyl-glycoluril. Biochem Biophys Res Commun 1978; 80: 849-857
    CrossrefPubMedGoogle Scholar
  • 27 Bertina RM, Wijngaarden A van, Reinalda-Poot JH, Poort SR, Bom V JJ. Determination of plasma protein S - the protein cofactor of activated protein C. Thromb Haemostas 1985; 53: 268-272
    PubMedGoogle Scholar
  • 28 Bertina RM, Broekmans AW, Linden IK van der, Mertens K. Protein C deficiency in a Dutch family with thrombotic disease. Thromb Haemostas 1982; 48: 1-5
    PubMedGoogle Scholar
  • 29 Bertina RM, Linden I K van der. Inhibitor neutralisation assay and electroimmunoassay of human factor IX (Christmas factor). Clin Chim Acta 1977; 77: 275-286
    CrossrefPubMedGoogle Scholar
  • 30 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantitaties of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254
    CrossrefPubMedGoogle Scholar
  • 31 Gjønnoes H. Cold promoted activation of factor VII. I. Evidence for the existence of an activator. Thrombos Diathes Haemorrh 1972; 28: 155-168
    PubMedGoogle Scholar
  • 32 Rao L VM, Bajaj SP, Rapaport SI. Activation of human factor VII during clotting in vitro. Blood 1985; 65: 218-226
    PubMedGoogle Scholar
  • 33 Mariani G, Mazzucconi MG. Factor VII congenital deficiency. Clinical picture and classification of the variants. Haemostasis 1983; 13: 169-177
    PubMedGoogle Scholar
  • 34 Meade TW, North W RS, Chakrabarti R, Stirling Y, Haines AP, Thompson SG. Haemostatic function and cardiovascular death: early results of a prospective study. Lancet 1980; 1: 1050-1054
    PubMedGoogle Scholar
  • 35 Miller GJ, Walter SJ, Stirling Y, Thompson SG, Esnouf MP. Assay of factor VII activity by two techniques: evidence for increased conversion of VII to a VIIa in hyperlipidaemia, with possible implications for ischaemic heart disease. Br J Haematol 1985; 59: 249-258
    CrossrefPubMedGoogle Scholar
  • 36 Van der Meer J, Hemker HC, Loeliger EA. Pharmacological aspects of vitamin K1 A clinical and experimental study in man. Thrombos Diathes Haemorrh 1968 29
    PubMedGoogle Scholar
  • 37 Poort SR, Linden IK van der, Krommenhoek-van Es C, Briët E, Bertina RM. Rabbit polyclonal antibodies against the calcium-dependent conformation of factor IX and their application in solid phase immunoradiometric assays. Thromb Haemostas 1986; 55: 122-128
    PubMedGoogle Scholar