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Thromb Haemost 1979; 42(02): 631-640
DOI: 10.1055/s-0038-1666902
Original Article
Schattauer GmbH Stuttgart

Platelet Aggregation Evoked In Vitro and In Vivo by Phosphatidic Acids and Lysoderivatives: Identity with Substances in Aged Serum (DAS)[*]

K A Schumacher
The Institute of Pharmacology, University of Freiburg and Department of Pharmacology, University of Hohenheim
,
H G Classen
The Institute of Pharmacology, University of Freiburg and Department of Pharmacology, University of Hohenheim
,
M Späth
The Institute of Pharmacology, University of Freiburg and Department of Pharmacology, University of Hohenheim
› Author Affiliations
Further Information

Publication History

Received 02 September 1977

Accepted 30 November 1977

Publication Date:
23 August 2018 (online)

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Summary

In serum incubated at 36° C for 18-24 hours a factor (DAS) develops which on intravenous injection into cats evokes platelet aggregation followed by an increase in pulmonary vascular resistance (PVR). This change in PVR is mediated via the platelets since it significantly correlates with the preinjection platelet count. There is evidence that phosphatidic acids (PA) and lysophosphatidic acids (LPA) are the active components of DAS. Investigations performed on platelet-rich plasma from man, cat, pig, dog, rabbit, guinea pig, and rat demonstrate that only human and feline platelets exposed to PA or to LPA are aggregated. Feline platelets are more sensitive to either compound than are the platelets from men; however, human platelets exhibit two exceptional properties, a) the sensitivity rapidly declines with time, b) pretreatment with subthreshold concentrations of LPA or PA induces a specific tachyphylaxis.

* Partially presented at the 1st Florence Conference on Thrombosis and Haemostasis, May 1977.


 

  • References

  • 1 Benner KU, Schumacher KA, Classen HG. 1974; Platelet aggregation induced by DAS in vitro: some investigation on its mode of action. Thrombosis et Diathesis Haemorrhagica 23: 354
    PubMedSearch in Google Scholar
  • 2 Benner KU, Schumacher KA, Classen HG. 1975; Platelet aggregation induced by anaphylatoxin and its inhibition. Arzneim.-Forsch 25: 1635
    PubMedSearch in Google Scholar
  • 3 Born GV R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology 162: 67
    PubMedSearch in Google Scholar
  • 4 Brecher G, Cronkite EP. 1950; Morphology and enumeration of human blood platelets. Journal of d. Applied Physiology 3: 365
    PubMedSearch in Google Scholar
  • 5 Hagedorn M, Schumacher KA, Sugihara H, Mtitermayer Chr. 1974; Morphological study of circulatory failure induced by a platelet aggregating factor DAS Beitr. Path 152: 105
    PubMedSearch in Google Scholar
  • 6 Marquardt P. 1961; Zur pyrogenen Wirkung von DAS (Frühgift). Helvetia Physiologia Acta 19 C 29
    PubMedSearch in Google Scholar
  • 7 Schumacher KA, Classen HH. 1972; Platelet aggregation and increased pulmonary vascular resistance in cats induced by DAS and ADP. Naunyn-Schmiedeberg’s Archives of Pharmacology 275: 373
    PubMedSearch in Google Scholar
  • 8 Schumacher KA, Classen HG, Hagedorn M, Benner KU, Spath M, Mtitermayer Chr. 1974; Effect of anaphylatoxin plasma in cats: hemodynamic changes induced by platelet aggregation. Arzneim.-Forsch 24: 122
    PubMedSearch in Google Scholar
  • 9 Wadsworth A, Maltaner F, Maltaner E. 1927; A study of coagulation of the blood; the chemical reactions underlying the process. American Journal of Physiology 80: 502
    PubMedSearch in Google Scholar