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Thromb Haemost 1979; 42(02): 694-704
DOI: 10.1055/s-0038-1666908
Original Article
Schattauer GmbH Stuttgart

Relationship Between Mepacrine-Labelled Dense Body Number, Platelet Capacity to Accumulate 14C-5-HT and Platelet Density in the Bernard-Soulier and Hermansky-Pudlak Syndromes

F Rendu
The Unité 150 INSERM et ERA 335 CNRS, Hôpital Lariboisière, 75475 Paris Cedex 10 (France)
,
A T Nurden
The Unité 150 INSERM et ERA 335 CNRS, Hôpital Lariboisière, 75475 Paris Cedex 10 (France)
,
M Lebret
The Unité 150 INSERM et ERA 335 CNRS, Hôpital Lariboisière, 75475 Paris Cedex 10 (France)
,
J P Caen
The Unité 150 INSERM et ERA 335 CNRS, Hôpital Lariboisière, 75475 Paris Cedex 10 (France)
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Publikationsverlauf

Received 11. Juli 1978

Accepted 20. Oktober 1978

Publikationsdatum:
23. August 2018 (online)

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Summary

We have used the mepacrine-labelling procedure to measure the dense body (serotonin storage organelle) content of the platelets of 2 hereditary disorders where abnormalities in dense body number were suspected. The platelets were incubated with mepacrine and examined by fluorescence microscopy. A mean number of 5.4 ± 0.8 (SD) dense bodies per platelet was calculated from the data obtained using platelets isolated from 40 normal human subjects. In contrast the platelets of 2 patients with the Bernard-Soulier syndrome contained an average of 14 and 17 labelled granules. This increase was associated with a much greater capacity of the platelets to accumulate 14C-5-HT. The opposite result was obtained using the platelets from 2 patients with the Hermansky-Pudlak syndrome which contained few granules labelled by mepacrine and took up less 14C-5-HT than normal human platelets. Centrifugation of the patients’ platelets on discontinuous sucrose gradients showed that the platelets of the 2 Bemard-Soulier patients were much denser than normal whereas a high proportion of low density platelets was observed in the Hermansky-Pudlak syndrome. These results further define the platelet abnormalities in the two syndromes and suggest that dense body number may be one of the factors governing platelet density.

 

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