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Thromb Haemost 1979; 42(02): 778-793
DOI: 10.1055/s-0038-1666916
Original Article
Schattauer GmbH Stuttgart

The Effects of Citrate and Extracellular Calcium Ions on the Platelet Release Reaction Induced by Adenosine Diphosphate and Collagen

Stanley Hepinstall
The Department of Medicine, University Hospital, Queen’s Medical Centre, Nottingham, NG7 2UH England
,
Patricia M Taylor
The Department of Medicine, University Hospital, Queen’s Medical Centre, Nottingham, NG7 2UH England
› Author Affiliations
Further Information

Publication History

Received 04 July 1978

Accepted 19 January 1979

Publication Date:
23 August 2018 (online)

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Summary

The ADP-induced release of 3H-serotonin from human platelets in heparinized platelet rich plasma is markedly stimulated by the addition of sodium citrate. The aggregation and release that is induced by collagen is less affected by citrate. Data is presented that supports the view that the effects of citrate on both ADP-and collagen-induced release are largely via alteration of the concentration of ionized calcium in plasma.

Collagen can induce release of 3H-serotonin via extracellular calcium-independent and -dependent mechanisms. The possibility that the calcium-dependent mechanism is aggregation-dependent and that the calcium is required for platelet aggregation rather than directly involved in the release reaction is discussed.

 

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