Monocytes of Different Subsets in Complexes with Platelets in Patients with Myocardial Infarction

Marina Loguinova; Natalia Pinegina; Valeria Kogan; Murad Vagida; Anush Arakelyan; Alexander Shpektor; Leonid Margolis; Elena Vasilieva

doi:10.1055/s-0038-1673342

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2018; 118(11): 1969-1981
DOI: 10.1055/s-0038-1673342

Atherosclerosis and Ischaemic Disease

Georg Thieme Verlag KG Stuttgart · New York

Monocytes of Different Subsets in Complexes with Platelets in Patients with Myocardial Infarction

Marina Loguinova^*

¹Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia

,

Natalia Pinegina^*

¹Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia

,

Valeria Kogan

²Ariel University, Institute for Translational Research, Ariel, Israel

,

Murad Vagida

¹Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia

,

Anush Arakelyan

³Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States

,

Alexander Shpektor

¹Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia

,

Leonid Margolis

³Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States

,

Elena Vasilieva

¹Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia

› Author Affiliations

Abstract

Acute myocardial infarction (AMI) is associated with activation of various cells, including platelets that form monocyte–platelet complexes (MPCs). Here, we analysed MPC in vivo and in vitro and investigated the abilities of different monocyte subclasses to form MPC, the characteristics of the cells involved in MPC formation and MPC changes in AMI. We identified MPC by co-staining for platelet antigen CD41a and monocyte antigens CD14 and CD16. Platelet activation was evaluated from expression of phosphatidylserine as revealed by annexin V. Our results confirm published data and provide new information regarding the patterns of MPC in AMI patients. We found that the patterns of platelet aggregation with monocytes were different in AMI patients and controls: (1) in AMI patients, MPC formed by intermediate monocytes carry more platelets whereas in healthy controls more platelets aggregated with classical monocytes; (2) the numbers of MPC in AMI patients, being already higher than in controls, were further increased if these patients suffered various in-hospital complications; (3) on the basis of the CD41a fluorescence of the antibody-stained MPC, some of the aggregates seem to consist of monocytes and platelet-derived extracellular vesicles (EVs); (4) aggregation of monocytes with platelet EV occurred in in vitro experiments; and (5) these experiments demonstrated that monocytes from AMI patients aggregate with both platelets and platelet EVs more efficiently than do monocytes from controls. MPC in AMI patients may play an important role in this pathology.

Keywords

monocyte subsets - monocyte–platelet complexes - extracellular vesicles - acute myocardial infarction - flow cytometry

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References

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Supplementary Material

Supplementary Material