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DOI: 10.1055/s-0038-1676318
Surface Acoustic Wave-Based Microfluidic Coagulation Device for Monitoring Anticoagulant Therapy
Funding Federal Ministry of Economics and Technology, grant number: FKZ 03SHWB027.Federal Ministry of Education and Research Project “On-Chip Flusskammer,” FKZ: 01EZ0850.
Publication History
Publication Date:
19 December 2018 (online)
![](https://www.thieme-connect.de/media/sth/201903/lookinside/thumbnails/10-1055-s-0038-1676318_02579-1.jpg)
Abstract
An universal coagulation test that reliably detects prolonged coagulation times in patients, regardless of which anticoagulant is administered, is not yet available. The authors developed a novel, miniaturized device utilizing surface acoustic waves (SAW) to detect clotting, and used it to develop a novel universal microfluidic coagulation test. Results from this assay were compared with results from standard coagulation assays to detect classical anticoagulants (unfractionated heparin, argatroban) and direct oral anticoagulants (dabigatran, rivaroxaban). The SAW-clotting time (SAW-CT) of this novel device was prolonged in a dose-dependent manner for heparin, argatroban, dabigatran, and rivaroxaban, comparable to standard assays. The authors confirmed the clinical utility of this device in a small patient population admitted to a stroke unit. Preliminary clinical data prove the suitability of the SAW-CT in patients receiving warfarin, rivaroxaban, or dabigatran. The device could be particularly useful as a point-of-care test to assess whole blood coagulation (e.g., in stroke units or in other emergency settings).
Patents
DMPA patent number 10 2011 001 952.
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References
- 1 Lindhoff-Last E, Ansell J, Spiro T, Samama MM. Laboratory testing of rivaroxaban in routine clinical practice: when, how, and which assays. Ann Med 2013; 45 (5-6): 423-429
- 2 Bouget J, Oger E. Emergency admissions for major haemorrhage associated with direct oral anticoagulants. Thromb Res 2015; 136 (06) 1190-1194
- 3 Rizos T, Horstmann S, Jenetzky E. , et al. Oral anticoagulants--a frequent challenge for the emergency management of acute ischemic stroke. Cerebrovasc Dis 2012; 34 (5-6): 411-418
- 4 Van Cott EM, Laposata M. Coagulation. In: Jacobs DS, Oxley DK, DeMott WR, eds. The Laboratory Test Handbook. 5th ed. Cleveland, OH: Lexi-Comp; 2001: 327-358
- 5 Meyer Dos Santos S, Zorn A, Guttenberg Z. , et al. A novel μ-fluidic whole blood coagulation assay based on Rayleigh surface-acoustic waves as a point-of-care method to detect anticoagulants. Biomicrofluidics 2013; 7 (05) 56502
- 6 Moll J, Meyer dos Santos S, Hils B. , et al. Micro-optical prototyping of a surface acoustic wave-based point-of-care coagulation assay and first application in anticoagulated patients. Int J Clin Pharmacol Ther 2016; 54 (03) 177-184
- 7 Bluecher A, Meyer Dos Santos S, Ferreirós N. , et al. Microfluidic coagulation assay for monitoring anticoagulant therapy in acute stroke patients. Thromb Haemost 2017; 117 (03) 519-528
- 8 Schneider SW, Nuschele S, Wixforth A. , et al. Shear-induced unfolding triggers adhesion of von Willebrand factor fibers. Proc Natl Acad Sci U S A 2007; 104 (19) 7899-7903
- 9 Steiner T, Böhm M, Dichgans M. , et al. Recommendations for the emergency management of complications associated with the new direct oral anticoagulants (DOACs), apixaban, dabigatran and rivaroxaban. Clin Res Cardiol 2013; 102 (06) 399-412