A Genome-Wide Analysis of Clinical Chorioamnionitis among Preterm Infants

Ariana M. Spiegel; Jingjing Li; John W. Oehlert; Jonathan A. Mayo; Cecele C. Quaintance; Anna I. Girsen; Maurice L. Druzin; Yasser Y. El-Sayed; Gary M. Shaw; David K. Stevenson; Ronald S. Gibbs

doi:10.1055/s-0038-1677503

American Journal of Perinatology, Inhaltsverzeichnis

Am J Perinatol 2019; 36(14): 1453-1458
DOI: 10.1055/s-0038-1677503

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

A Genome-Wide Analysis of Clinical Chorioamnionitis among Preterm Infants

Ariana M. Spiegel

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

,

Jingjing Li

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

John W. Oehlert

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

Jonathan A. Mayo

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

Cecele C. Quaintance

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

Anna I. Girsen

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

,

Maurice L. Druzin

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

,

Yasser Y. El-Sayed

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

,

Gary M. Shaw

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

David K. Stevenson

²Division of Neonatal and Developmental Medicine, Department of Pediatrics, March of Dimes Prematurity Research Center at Stanford University, Stanford University School of Medicine, Stanford, California

,

Ronald S. Gibbs

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California

› Institutsangaben

Abstract

Objective To identify single nucleotide polymorphisms (SNPs) associated with clinical chorioamnionitis among preterm infants.

Study Design We reanalyzed a genome-wide association study (GWAS) from preterm newborns at less than 30 weeks' gestation. Cases and control definitions were determined using administrative records. There were 213 clinical chorioamnionitis cases and 707 clinically uninfected controls. We compared demographic and clinical outcomes of cases and controls. We performed a GWAS and compared the distribution of SNPs from the background genes and from the immunome genes. We used a Wilcoxon's rank-sum test to compare the SNPs normalized odds ratio and used odds ratios and p-values to determine candidate genes.

Results Infants affected by clinical chorioamnionitis were more likely to have periventricular leukomalacia, high-grade retinopathy, and high-grade intraventricular hemorrhage (IVH). Although a GWAS did not identify SNPs associated with clinical chorioamnionitis at the genome-wide significance level, a direct test on the exonic variants in the human immunome revealed their significant increase of risk in clinical chorioamnionitis.

Conclusion Among very preterm infants, clinical chorioamnionitis was associated with periventricular leukomalacia, high-grade retinopathy, and IVH. Our analysis of variants in the human immunome indicates an association with clinical chorioamnionitis in very preterm pregnancies.

Keywords

candidate gene - analysis - clinical chorioamnionitis - genome-wide association study - preterm birth

Volltext

Referenzen

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