Approaches to the pathognomonic endotype definition of chronic rhinosinusitis based on the expression of cell adhesion proteins

Introduction:

An understanding of the pathophysiology of the sinunasal mucosa in chronic rhinosinusitis provides the basis for new treatment modalities. Cell adhesion molecules ensure epithelial integrity. In previous studies, we identified the most striking changes in protein expression of the sinunasal epithelial barrier.

Methods:

Sinonasal tissue, as well as tissues from the lower turbinate (control cohort) of patients (n = 46) with chronic rhinosinusitis with and without polyps, were removed intraoperatively. Several tissue microarrays (TMA) were prepared on the tissue and coated with fluorescently labeled antigens against desmoglein 2. Representative sections were taken with a Kodak microscope under a TRITC filter. Expression was assessed by visual analog scale and correlated to the level of interleukin 5 concentration of tissue types.

Results:

There were highly significant changes in the expressivity of desmoglein 2 (p < 0.001) as well as interleukin 5 (p < 0.001) in the group compared between tissue with chronic rhinosinusitis and control tissue.

Conclusion:

This study is intended to further define the definition of endotypes and to identify immunopathological processes in chronic rhinosinusitis, which may contribute to the understanding of chronic rhinosinusitis with and without polyps.

Publication History

Publication Date:
23 April 2019 (online)