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CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(03): 318-322
DOI: 10.1055/s-0039-1697117
Original Article
Dental Investigation Society

Influence of Thymoquinone Exposure on the Micro-Hardness of Dental Enamel: An In Vitro Study

Imran Farooq
1   Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
,
Saqib Ali 
1   Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
,
Intisar Ahmad Siddiqui
2   Department of Quality Assurance, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
,
Khalifa S. Al-Khalifa
3   Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
,
Mohammed Al-Hariri
4   Department of Physiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
› Author Affiliations
Further Information

Publication History

Publication Date:
16 October 2019 (online)

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Abstract

Objective The aim of this study was to assess changes in micro-hardness level of enamel after it was exposed to thymoquinone (TQ).

Materials and Methods Sixteen enamel blocks were prepared and divided into two groups (each group received eight blocks, n = 8); Gp 1 (control): enamel blocks kept in 100 mL artificial saliva (AS) for 24 hours and Gp 2: enamel blocks kept in a mixture of TQ powder (1 g) and AS (100 mL) for 24 hours. Post-immersion they were subjected to simulated brushing with each sample receiving 8,000 linear strokes. For brushing, 3 mL of AS and TQ oil was used for groups 1 and 2, respectively. Enamel surfaces were analyzed for changes in values of surface micro-hardness (pre-immersion, post-immersion, and post-brushing) by obtaining Vickers hardness number (VHN).

Results The present study indicated improvement in micro-hardness levels for both groups although experimental group showed more enhancement. The mean baseline VHN for control group was 498.6, 500.4 for post-immersion, and 503.5 for post-brushing. The mean baseline VHN for experimental group was 448.7, 531 for post-immersion, and 610.3 for post-brushing. Statistically significant differences (p < 0.05) were observed when post-brushing VHN values of both groups were compared and also within the experimental group when post-brushing values were compared with baseline values.

Statistical Analysis Wilcoxon signed-rank test was applied for the evaluation of pre- and post-exposure hardness values. Level of significance was ≤0.05.

Conclusion The exposure of enamel to TQ led to an improvement in its micro-hardness levels. Further studies are required to understand the mechanism of action of TQ on human tissues.

Keywords

enamel - micro-hardness - Nigella sativa - thymoquinone
 

  • References

  • 1 Dewhirst FE, Chen T, Izard J. et al. The human oral microbiome. J Bacteriol 2010; 192 (19) 5002-5017
    CrossrefPubMedSearch in Google Scholar
  • 2 Kuboniwa M, Tribble GD, Hendrickson EL, Amano A, Lamont RJ, Hackett M. Insights into the virulence of oral biofilms: discoveries from proteomics. Expert Rev Proteomics 2012; 9 (03) 311-323
    CrossrefPubMedSearch in Google Scholar
  • 3 Zafar MS, Ahmed N. Nano-mechanical evaluation of dental hard tissues using indentation technique. World Appl Sci J 2013; 28 (10) 1393-1399
    Search in Google Scholar
  • 4 Zafar MS, Ahmed N. The effects of acid etching time on surface mechanical properties of dental hard tissues. Dent Mater J 2015; 34 (03) 315-320
    CrossrefPubMedSearch in Google Scholar
  • 5 Alfaroukh R, ElEmbaby A, Almas K. et al. Oral biofilm formation and retention on commonly used dental materials: an update. Odontostomatol Trop 2018; 41 (164) 28-34
    Search in Google Scholar
  • 6 Alhussain AM, Alhaddad AA, Ghazwi MM, Farooq I. Remineralization of artificial carious lesions using a novel fluoride incorporated bioactive glass dentifrice. Dent Med Probl 2018; 55 (04) 379-382
    CrossrefPubMedSearch in Google Scholar
  • 7 Zero DTFM, Fontana M, Martínez-Mier EA. et al. The biology, prevention, diagnosis and treatment of dental caries: scientific advances in the United States. J Am Dent Assoc 2009; 140 (Suppl. 01) 25S-34S
    CrossrefPubMedSearch in Google Scholar
  • 8 Lemos JA, Quivey Jr. RG, Koo H, Abranches J. Streptococcus mutans: a new gram-positive paradigm?. Microbiology 2013; 159 (Pt 3) 436-445
    Search in Google Scholar
  • 9 Raja M, Hannan A, Ali K. Association of oral candidal carriage with dental caries in children. Caries Res 2010; 44 (03) 272-276
    PubMedSearch in Google Scholar
  • 10 Rouabhia M, Chmielewski W. Diseases associated with oral polymicrobial biofilms. Open Mycol J. 2012; 6: 27-32
    CrossrefSearch in Google Scholar
  • 11 Al-Attass SA, Zahran FM, Turkistany SA. Nigella sativa and its active constituent thymoquinone in oral health. Saudi Med J 2016; 37 (03) 235-244
    CrossrefPubMedSearch in Google Scholar
  • 12 Rahmani AH, Aly SM. Nigella Sativa and its active constituents thymoquinone shows pivotal role in the diseases prevention and treatment. Asian J Pharm Clin Res. 2015; 8 (01) 48-53
    Search in Google Scholar
  • 13 Gaur S, Shrivastava B, Gaur S, Bhardwaj R, Khanchandani R. Medicinal and therapeutical potential of Nigella sativa. Int J Med App Sci Res 2014; 1: 32-39
    Search in Google Scholar
  • 14 Ahmad A, Husain A, Mujeeb M. et al. A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed 2013; 3 (05) 337-352
    PubMedSearch in Google Scholar
  • 15 Padhye S, Banerjee S, Ahmad A, Mohammad R, Sarkar FH. From here to eternity - the secret of Pharaohs: therapeutic potential of black cumin seeds and beyond. Cancer Ther 2008; 6b: 495-510
    Search in Google Scholar
  • 16 Ahmad I, Tripathi J, Sharma M, Karchulli MS, Umer L. Nigella sativa - a medicinal herb with immense therapeutic potential (a systematic review). Int J Bio Pharm Res 2014; 5: 755-762
    Search in Google Scholar
  • 17 Shaker A, Al-Wafi H. Benefits of thymoquinone, a Nigella Sativa extract in preventing dental caries initiation and improving gingival health. ProQuest 2014; LLC 72
    Search in Google Scholar
  • 18 Al-Douri A, Al-Kazaz S. The effect of Nigella Sativa oil (black seed) on the healing of chemically induced oral ulcer in rabbit (experimental study). Al-Rafidain Dent J 2010; 10: 151-157
    Search in Google Scholar
  • 19 Al-Thobity AM, Al-Khalifa KS, Gad MM, Al-Hariri M, Ali AA, Alnassar T. In vitro evaluation of the inhibitory activity of thymoquinone in combatting Candida albicans in denture stomatitis prevention. Int J Environ Res Public Health 2017; 14 (07) 743
    CrossrefPubMedSearch in Google Scholar
  • 20 Fusayama T, Katayori T, Nomoto S. Corrosion of gold and amalgam placed in contact with each other. J Dent Res 1963; 42: 1183-1197
    CrossrefPubMedSearch in Google Scholar
  • 21 Farooq I, Moheet IA, AlShwaimi E. In vitro dentin tubule occlusion and remineralization competence of various toothpastes. Arch Oral Biol 2015; 60 (09) 1246-1253
    CrossrefPubMedSearch in Google Scholar
  • 22 Zafar MS. A comparison of dental restorative materials and mineralized dental tissues for surface nanomechanical properties. Life Sci J 2014; 1110s: 19-24
    Search in Google Scholar
  • 23 Gutiérrez-Salazar M, Reyes-Gasga J. Microhardness and chemical composition of human tooth. Mater Res 2003; 6 (03) 367-373
    CrossrefSearch in Google Scholar
  • 24 Hassan U, Farooq I, Moheet IA, AlShwaimi E. Cutting efficiency of different dental materials utilized in an air abrasion system. Int J Health Sci (Qassim) 2017; 11 (04) 23-27
    Search in Google Scholar
  • 25 Shaikh K, Pereira R, Gillam DG, Phad S. Comparative evaluation of desensitizing dentifrices containing BioMin®, Novamin® and fluoride on dentinal tubule occlusion before and after a citric acid challenge– a scanning electron microscope in-vitro Study. J Odontol 2018; 2 (01) 105
    Search in Google Scholar
  • 26 Zafar MS, Ahmed N. Nanomechanical characterization of exfoliated and retained deciduous incisors. Technol Health Care 2014; 22 (06) 785-793
    PubMedSearch in Google Scholar
  • 27 Alencar CR, Mendonça FL, Guerrini LB. et al. Effect of different salivary exposure times on the rehardening of acid-softened enamel. Braz Oral Res 2016; 30 (01) e104
    PubMedSearch in Google Scholar
  • 28 Wang X, Mihailova B, Klocke A, Heidrich S, Bismayer U. Effect of artificial saliva on the apatite structure of eroded enamel. Int J Spectrosc 2011. 2011 Available at: http://www.hindawi.com/journals/ijs/2011/236496/.doi:10.1155/2011/236496
    Search in Google Scholar
  • 29 Lebwohl M, Ali S. Treatment of psoriasis. Part 2. Systemic therapies. J Am Acad Dermatol 2001; 45 (05) 649-661, quiz 662–664
    CrossrefPubMedSearch in Google Scholar
  • 30 Kishwar F, Mahmood T, Mahmood I, Anwar A, Parween R, Mustafa S. Complexation of active ingredient thymoquinone of nigella sativa (black seed) with chromium(vi). Fuuast J BIOL. 2016; 6 (01) 65-72
    Search in Google Scholar
  • 31 Ermumcu MSK, Sanher N. Black cumin (Nigella sativa) and its active component of thymoquinone: effects on health. J Food Health Sci 2017; 3 (04) 170-183
    Search in Google Scholar
  • 32 Mehta BK, Pandit V, Gupta M. New principles from seeds of Nigella sativa. Natural product research. Part A 2009; 23: 138-148
    Search in Google Scholar
  • 33 Akram Khan M, Afzal M. Chemical composition of Nigella sativa Linn: Part 2 recent advances. Inflammopharmacology 2016; 24 (02) (03) 67-79
    CrossrefPubMedSearch in Google Scholar
  • 34 Khazdair MR. The protective effects of Nigella sativa and its constituents on induced neurotoxicity. J Toxicol 2015; 2015: 841823
    Search in Google Scholar
  • 35 Beheshti F, Khazaei M, Hosseini M. Neuropharmacological effects of Nigella sativa. Avicenna J Phytomed 2016; 6 (01) 104-116
    PubMedSearch in Google Scholar
  • 36 Darakhshan S, Bidmeshki Pour A, Hosseinzadeh Colagar A, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacol Res 2015; 95-96: 138-158
    CrossrefPubMedSearch in Google Scholar
  • 37 El-Dakhakhny M, Madi NJ, Lembert N, Ammon HP. Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats. J Ethnopharmacol 2002; 81 (02) 161-164
    CrossrefPubMedSearch in Google Scholar