PDF herunterladen
CC BY-NC-ND 4.0 · Eur J Dent 2009; 03(02): 100-106
DOI: 10.1055/s-0039-1697415
Original Article
Dental Investigation Society

Increased Levels of 8-Hydroxydeoxyguanosine and Malondialdehyde and its Relationship with Antioxidant Enzymes in Saliva of Periodontitis Patients

Cenk Fatih Canakci
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
,
Yasin Cicek
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
,
Abdulkadir Yildirim
b   Department of Biochemistry, Ataturk University, Faculty of Medicine, Erzurum, Turkey
,
Ufuk Sezer
c   Department of Periodontology, Karadeniz Technical University, Faculty of Dentistry, Trabzon, Turkey
,
Varol Canakci
a   Department of Periodontology, Ataturk University, Faculty of Dentistry, Erzurum, Turkey
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
27. September 2019 (online)

Auch verfügbar auf
  als PDF herunterladen Lizenzen und Reprints

ABSTRACT

Objectives: The aim of this study was to evaluate 8-hydroxydeoxyguanosine (8-OHdG) and Malondialdehyde (MDA) levels, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in whole saliva of patients with chronic periodontitis. Moreover, the relationship among the oxidative damage biomarkers, antioxidant enzymes activities and clinical periodontal status were investigated.

Methods: Whole saliva samples were collected from 30 patients with chronic periodontitis and 30 periodontally healthy control. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level, and probing depth were measured. The salivary 8-OHdG level was measured using the ELISA method. SOD and GPx activities and MDA levels were determined spectrophotometrically.

Results: Higher salivary 8-OHdG and MDA levels (P<.001), and lower salivary SOD and GPx activities (P<.05) were detected in periodontitis patients compared to the healthy controls. Additionally, there were significantnegative correlations between salivary levels of 8-OHdG and both salivary SOD and GPx activities as well as between salivary levels of MDA and both salivary SOD and GPx activities (P<.001).

Conclusions: Higher salivary 8-OHdG and MDA levels and lower salivary antioxidant activities seem to reflect increased oxygen radical activity during periodontal inflammation. (Eur J Dent 2009;3:100- 106)

Key words:

8-hydroxydeoxyguanosine - Malondialdehyde - Superoxide dismutase - Glutathione peroxidase - Chronic periodontitis - Saliva
 

  • REFERENCES

  • 1 Sies H. Oxidative stress: Oxidants and Antioxidants. Academic Press; New York: 1991
    Google Scholar
  • 2 Canakci CF, Cicek Y, Canakci V. Reactive oxygen species and human inflammatory periodontal diseases. Biochemistry (Mosc) 2005; 70: 619-628
    Google Scholar
  • 3 Miller DR, Lamster IB, Chasens AI. Role of the polymorphonuclear leukocyte in periodontal health and disease. J Clin Periodontol 1984; 11: 1-15
    CrossrefPubMedGoogle Scholar
  • 4 Moseley R, Waddington RJ, Embery G. Degradation of glycosaminoglycans by reactive oxygen species derived from stimulated polymorphonuclear leukocytes. Biochim Biophys Acta 1997; 1362: 221-231
    CrossrefPubMedGoogle Scholar
  • 5 Sculley DV, Langley-Evans SC. Salivary antioxidants and periodontal disease status. Proc Nutr Soc 2002; 61: 137-143
    CrossrefPubMedGoogle Scholar
  • 6 Ozmeric N. Advances in periodontal disease markers. Clin Chim Acta 2005; 343: 1-16
    Google Scholar
  • 7 Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000 2007; 43: 160-232
    CrossrefPubMedGoogle Scholar
  • 8 Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu JT. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta 2003; 334: 87-94
    CrossrefPubMedGoogle Scholar
  • 9 Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta 2004; 339: 1-9
    CrossrefPubMedGoogle Scholar
  • 10 Onorato JM, Thorpe SR, Baynes JW. Immunohistochemical and ELISA assays for biomarkers of oxidative stress in aging and disease. Ann N Y Acad Sci 1998; 20 (854) 277-290
    Google Scholar
  • 11 Richter C. Biophysical consequences of lipid peroxidation in membranes. Chem Phys Lipids 1987; 44: 175-189
    CrossrefPubMedGoogle Scholar
  • 12 Matés JM, Pérez-Gómez C, Nûnez de Castro I. Antioxidant enzymes and human diseases. Clin Biochem 1999; 32: 595-603
    CrossrefPubMedGoogle Scholar
  • 13 Halliwell B. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 1991; 91(3C): 14-22
    Google Scholar
  • 14 Fattman CL, Schaefer LM, Oury TD. Extracellular superoxide dismutase in biology and medicine. Free Radic Biol Med 2003; 35: 236-256
    CrossrefPubMedGoogle Scholar
  • 15 Tibell L, Aasa R, Marklund SL. Spectral and physical properties of human extracellular superoxide dismutase: A comparison with CuZn superoxide dismutase. Arch Biochem Biophys 1993; 304: 429-433
    CrossrefPubMedGoogle Scholar
  • 16 Maxwell SR. Prospects for the use of antioxidant therapies. Drugs 1995; 49: 345-361
    CrossrefPubMedGoogle Scholar
  • 17 Sies H, Sharov VS, Klotz LO, Briviba K. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase. J Biol Chem 1997; 272: 27812-27817
    CrossrefPubMedGoogle Scholar
  • 18 Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999; 4: 1-6
    CrossrefPubMedGoogle Scholar
  • 19 Silness J, Löe H. Periodontal disease in pregnancyII Correlation of between oral hygiene and periodontal condition. Acta Odontol Scand 1964; 22: 121-135
    CrossrefPubMedGoogle Scholar
  • 20 Löe H, Silness J. Periodontal disease in pregnancy I. Prevalance and severity. Acta Odontol Scand 1963; 21: 531-551
    Google Scholar
  • 21 Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-169
    PubMedGoogle Scholar
  • 22 Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500
    CrossrefPubMedGoogle Scholar
  • 23 Jain SK, McVie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes 1989; 38: 1539-1543
    CrossrefPubMedGoogle Scholar
  • 24 Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2’-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 1997; 387: 147-163
    PubMedGoogle Scholar
  • 25 Takane M, Sugano N, Iwasaki H, Iwano Y, Shimizu N, Ito K. New biomarker evidence of oxidative DNA damage in whole saliva from clinically healthy and periodontally diseased individuals. J Periodontol 2002; 73: 551-554
    CrossrefPubMedGoogle Scholar
  • 26 Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I. Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 2006; 73: 252-260
    CrossrefPubMedGoogle Scholar
  • 27 Bahar G, Feinmesser R, Shpitzer T, Popovtzer A, Nagler RM. Salivary analysis in oral cancer patients: DNA and protein oxidation, reactive nitrogen species, and antioxidant profile. Cancer 2007; 109: 54-59
    CrossrefPubMedGoogle Scholar
  • 28 Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem 1997; 43: 1209-1214
    CrossrefPubMedGoogle Scholar
  • 29 Khalili J, Biloklytska H. Salivary malondialdehyde levels in clinically healthy and periodontal diseased individuals. Oral Dis 2008; 14: 754-760
    CrossrefPubMedGoogle Scholar
  • 30 Eisenberg E, Shtahl S, Geller R, Reznick AZ, Sharf O, Ravbinovich M, Erenreich A, Nagler RM. Serum and salivary oxidative analysis in Complex Regional Pain Syndrome. Pain 2008; 138: 226-232
    CrossrefPubMedGoogle Scholar
  • 31 Saral Y, Coskun BK, Ozturk P, Karatas F, Ayar A. Assessment of salivary and serum antioxidant vitamins and lipid peroxidation in patients with recurrent aphthous ulceration. Tohoku J Exp Med 2005; 206: 305-312
    CrossrefPubMedGoogle Scholar
  • 32 Sawamoto Y, Sugano N, Tanaka H, Ito K. Detection of periodontopathic bacteria and an oxidative stress marker in saliva from periodontitis patients. Oral Microbiology Immunology 2005; 20: 216-220
    CrossrefPubMedGoogle Scholar
  • 33 Guentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E, Sigusch BW. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clin Oral Investig 2008; 12: 345-352
    CrossrefPubMedGoogle Scholar
  • 34 Sonoki K, Nakashima S, Takata Y, Naito T, Fujisawa K, Ootsubo T, Wakisaka M, Iwase M, Iida M, Yokota M. Decreased lipid peroxidation following periodontal therapy in type 2 diabetic patients. J Periodontol 2006; 77: 1907-1913
    CrossrefPubMedGoogle Scholar
  • 35 Canakci V, Yildirim A, Canakci CF, Eltas A, Cicek Y, Canakci H. Total antioxidant capacity and antioxidant enzymes in serum, saliva, and gingival crevicular fluid of preeclamptic women with and without periodontal disease. J Periodontol 2007; 78: 1602-1611
    CrossrefPubMedGoogle Scholar
  • 36 Marnett LJ. Oxyradicals and DNA damage. Carcinogenesis 2000; 21: 361-370
    CrossrefPubMedGoogle Scholar
  • 37 Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 1991; 11: 81-128
    CrossrefPubMedGoogle Scholar
  • 38 Park JW, Floyd RA. Lipid peroxidation products mediate the formation of 8-hydroxydeoxyguanosine in DNA. Free Radic Biol Med 1992; 12: 245-250
    CrossrefPubMedGoogle Scholar
  • 39 Wong YT, Ruan R, Tay FE. Relationship between levels of oxidative DNA damage, lipid peroxidation and mitochondrial membrane potential in young and old F344 rats. Free Radic Res 2006; 40: 393-402
    CrossrefPubMedGoogle Scholar
  • 40 Panjamurthy K, Manoharan S, Ramachandran CR. Lipid peroxidation and antioxidant status in patients with periodontitis. Cell Mol Biol Lett 2005; 10: 255-264
    PubMedGoogle Scholar
  • 41 Akalin FA, Baltacioglu E, Alver A, Karabulut E. Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis. J Clin Periodontol 2007; 34: 558-565
    CrossrefPubMedGoogle Scholar
  • 42 Brock GR, Butterworth CJ, Matthews JB, Chapple IL. Local and systemic total antioxidant capacity in periodontitis and health. J Clin Periodontol 2004; 31: 515-521
    CrossrefPubMedGoogle Scholar
  • 43 Mashayekhi F, Aghahoseini F, Rezaie A, Zamani MJ, Khorasani R, Abdollahi M. Alteration of cyclic nucleotides levels and oxidative stress in saliva of human subjects with periodontitis. J Contemp Dent Pract 2005; 6: 46-53
    CrossrefPubMedGoogle Scholar