RSS-Feed abonnieren
![](/products/assets/desktop/img/oa-logo.png)
DOI: 10.1055/s-0039-1697418
Cytotoxicity Evaluation of Self Adhesive Composite Resin Cements by Dentin Barrier Test on 3D Pulp Cellss
Publikationsverlauf
Publikationsdatum:
27. September 2019 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00042133/200902/lookinside/thumbnails/10-1055-s-0039-1697418_00120-1.jpg)
ABSTRACT
Objectives: The aim of this study was to evaluate the effects of five self-etch dental composite resin cements on the cell viability of bovine dental papilla-derived cells.
Methods: The cytotoxicity of composite resin cements (Rely X Unicem Clicker, 3M ESPE; MaxCem; KERR, PanaviaF 2.0; Kuraray, BisCem; Bisco and Bistite II DC; Tokuyama) was analyzed in a dentin barrier test device using three-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into two compartments by 500 im dentin disc. The three dimensional cultures placed on a dentin disk held in place by a special biocompatible stainless-steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer’s instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with medium (2 ml/h). After an exposure period of 24 h, the cell survival was determined by the MTT assay. Statistical analyses were performed using the Mann–Whitney U-test.
Results: In dentin barrier test, cell survival was similar with Maxcem and negative control group (P>.05), and all other tested materials were cytotoxic for the three dimensional cell cultures (P>.05).
Conclusions: The significance of composite resin cements is being more important in dentistry. The cytotoxic potencies demonstrated by these materials might be of clinical relevance. Some composite resin cements include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities or directly contact pulp tissue. (Eur J Dent 2009;3:120-126)
-
REFERENCES
- 1 El-Mowafy O. The use of resin cements in restorative dentistry to overcome retention problems. J Can Dent Assoc 2001; 67: 97-102
- 2 Craig RG. Restorative dental materials. 10th ed. St Louis: Mosby; 1997
- 3 Rosenstiel SF, Land MF, Crispin BJ. Dental luting agents: A review of the current literature. J Prosthet Dent 1998; 80: 280-301
- 4 Gerzina TM, Hume WR. Diffusion of monomers from bonding resin-resin composites combination through dentine in vitro. J Dent 1996; 24: 125-128
- 5 Geurtsen W, Spahl W, Muller K, Leyhausen G. Aqueous extracts from dentin adhesives contain cytotoxic chemical. J Biomed Mater Res Appl Biomater 1999; 48: 772-777
- 6 Costa CAS, Hebling J, Hanks CT. Current status of pulp capping with dentin adhesive systems: a review. Dent Mater 2000; 16: 188-197
- 7 Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G. et al Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater 2007; 23: 688-695
- 8 Demirci M, Hiller KA, Bosl C, Galler K, Schmalz G, Schweikl H. The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dent Mater 2008; 24: 362-371
- 9 Costa CAS, Nascimento ABL, Teixeira HM. Response of human pulps following acid conditioning and application of a bonding agent in deep cavities. Dent Mater 2002; 18: 543-551
- 10 Costa CAS, Hebling J, Randall RC. Human pulp response to resin cements used to bond inlay restorations. Dent Mater 2006; 22: 954-962
- 11 Moszner N, Ulrich S. New developments of polymeric dental composites. Prog Polym Sci 2001; 26: 535-576
- 12 Sideridou ID. Achilias DS Elution study of unreacted bis-GMA TEGDMA, UDMA, and bis-EMA from light-cured dental resins and resin composites using HPLC. J Biomed Mater Res B Appl Biomater 2005; 74: 617-626
- 13 Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res 1997; 37: 517-524
- 14 Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res 1998; 41: 474-480
- 15 Altintas SH, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res Part B: Appl Biomater 2008; 86B: 523-529
- 16 Ferracane JL. Elution of leachable components from composites. J Oral Rehabil 1994; 21: 441-452
- 17 Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: a review. Clin Oral Invest 2008; 12: 1-8
- 18 Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991; 70: 1450-1455
- 19 Jontell M, Hanks CT, Bratell JT, Bergenholtz G. Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp. J Dent Res 1995; 74: 1162-1167
- 20 de Mendonça AA, Souza PP, Costa CA. Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 104: 102-108
- 21 Schmalz G, Garhammer P, Schweikl H. A commercially available cell culture device modified for dentin barrier tests. J Endod 1996; 22: 249-252
- 22 Thonemann B, Schmalz G. Bovine dental papilla-derived cells immortalized with HPV 18 E6/E7. Eur J Oral Sci 2000; 108: 432-441
- 23 Schmalz G, Schuster U, Nützel K, Schweikl H. An in vitro pulp chamber with three-dimensional cell cultures. J Endod 1999; 25: 24-29
- 24 Schmalz G, Schuster U, Thonemann B, Barth M, Esterbauer S. Dentin barrier test with transfected bovine pulp-derived cells. J Endod 2001; 2: 96-102
- 25 Schuster U, Schmalz G, Thonemann B, Mendel N, Metzl C. Cytotoxicity testing with three-dimensional cultures of transfected pulp-derived cells. J Endod 2001; 27: 259-265
- 26 Galler K, Hiller KA, Ettl T, Schmalz G. Selective influence of dentin thickness upon cytotoxicity of dentin contacting materials. J Endod 2005; 31: 396-399
- 27 Schweikl H, Spagnuolo G, Schmalz G. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006; 85: 870-877
- 28 Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W. Effect of TEGDMA on the intracellular glutathione concentration of human gingival fibroblasts. J Biomed Mater Res 2002; 63: 746-751
- 29 Engelmann J, Janke V, Volk J, Leyhausen G, von Neuhoff N, Schlegelberger B, Geurtsen W. Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro. Biomaterials 2004; 25: 4573-4580
- 30 Santerre JP, Shajii L, Leung BW. Relation of dental composite formulations to their degradation and the release of hydrolyzed polymeric-resin-derived products. Crit Rev Oral Biol Med 2001; 12: 136-151
- 31 Michelsen VB, Lygre H, Skalevik R, Tveit AB, Solheim E. Identification of organic eluates from four polymer-based dental filling materials. Eur J Oral Sci 2003; 111: 263-271
- 32 Thonemann B, Schmalz G, Hiller K-A, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized dental papilla-derived cell lines to dental resin components. Dent Mater 2002; 18: 318-323
- 33 Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, Wang RS, Jeng JH. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 2005; 26: 745-753
- 34 Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, Coutinho E, Suzuki K, Lambrechts P, Van Meerbeek B. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007; 28: 3757-3785