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CC BY-NC-ND 4.0 · Eur J Dent 2009; 03(03): 191-199
DOI: 10.1055/s-0039-1697431
Original Article
Dental Investigation Society

Circulating β1 Adrenergic Autoantibodies from Patients with Chronic Periodontitis Interact with Gingival Fibroblasts

Leonor Sterin-Borda
a   Pharmacology Unit, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina
b   Argentine National Research Council (CONICET), Buenos Aires, Argentina
,
César Furlan
a   Pharmacology Unit, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina
,
Enri Borda
a   Pharmacology Unit, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina
b   Argentine National Research Council (CONICET), Buenos Aires, Argentina
› Author Affiliations
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Publication History

Publication Date:
27 September 2019 (online)

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ABSTRACT

Objectives: To demonstrate the presence of circulating autoantibodies (Abs) from patients with chronic periodontitis (CP) that interacted with human gingival fibroblast membranes activating β1 adrenoceptors (β1-AR).

Methods: Sera and purified IgG from 25 patients with CP and 20 age-matched healthy subjects were studied by flow cytometry, ELISA and DNA synthesis. Human gingival fibroblast membranes and/or synthetic peptides with amino acid sequences identical to human β1-AR were used as antigens.

Results: By flow cytometry and ELISA procedures, we proved that the serum-purified IgG fraction from patients with CP reacted with the fibroblast surface and to the β1 synthetic peptide. The corresponding affinity-purified anti-β1 peptide Abs displayed agonist-like activity associated with specific receptor activation, inhibiting the DNA synthesis of human gingival fibroblasts.

Conclusions: This study demonstrates that β1-AR autoantibodies are elevated in patients with CP. These autoantibodies were targeted to the fibroblasts, and specifically to the β1-AR, and has receptor-like activity inhibiting DNA synthesis. (Eur J Dent 2009;3:191-199)

Key words:

Cell proliferation - Cytokines - Autoantibodies - Antigens/peptides - Signal transduction - Gingival fibroblasts
 

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