CC BY-NC-ND 4.0 · Eur J Dent 2010; 04(04): 395-402
DOI: 10.1055/s-0039-1697859
Original Article
European Journal of Dentistry

Effectiveness of Two Methods for Preparation of Autologous Platelet-Rich Plasma: An Experimental Study in Rabbits

Maria J. H. Nagata
a   Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, UNESP, Brazil
,
Michel R. Messora
b   Division of Integrated Clinic, Dental School of Lavras, Lavras University Center, UNILAVRAS, Brazil
,
Flávia A. C. Furlaneto
a   Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, UNESP, Brazil
,
Stephen E. Fucini
a   Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, UNESP, Brazil
,
Alvaro F. Bosco
a   Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, UNESP, Brazil
,
Valdir G. Garcia
a   Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, UNESP, Brazil
,
Tatiana M. Deliberador
c   Program in Clinical Dentistry, Positivo University, Brazil
,
Luiz G. N. de Melo
d   Private Practice, Goiânia, Brazil
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Publikationsverlauf

Publikationsdatum:
30. September 2019 (online)

Objectives: The purpose of this study was to compare the quantity and quality of platelets in platelet- rich plasma (PRP) samples prepared using either the single- or the double-centrifugation protocol.

Methods: Ten adult white New Zealand rabbits were used. Ten ml of blood were drawn from each animal via cardiac puncture. Each blood sample was divided into two equal parts for PRP preparation: 5 ml of blood were centrifuged according to a single-centrifugation protocol (Group I), and 5 ml were centrifuged according to a double-centrifugation protocol (Group II). Manual platelet counts were performed on the whole blood and PRP samples of each group. Smears were also done on all samples in order to see the morphology of the platelets. The data obtained in the manual platelet count were submitted to statistical analysis (repeated measures ANOVA, Tukey, P<.05).

Results: The average whole blood platelet count was 446,389/μl. The PRP samples in Group II presented an average platelet amount significantly higher than that of Group I (1,986,875 ± 685,020/μl and 781,875 ± 217,693/μl, respectively). The PRP smears from Group II were the only one to present platelets with altered morphology (75% of the smears). A few lymphocytes with increased cytoplasm were observed in the PRP smears of both Groups I (25% of the smears) and II (62.5% of the smears).

Conclusions: Within the limits of this study, it can be concluded that the double-centrifugation protocol resulted in higher platelet concentrations than did the single-centrifugation protocol. However, the double-centrifugation protocol caused alterations in platelet morphology and was more sensitive to small processing errors. (Eur J Dent 2010;4:395-402)

 
  • REFERENCES

  • 1 Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004; 62: 489-496
  • 2 Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of sites for implants. Int J Oral Maxillofac Implants 1999; 14: 529-535
  • 3 Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001; 107: 229-237
  • 4 Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: a reentry study. J Periodontol 2002; 73: 198-205
  • 5 Hanna R, Trejo PM, Weltman RL. Treatment of intrabony defects with bovine-derived xenograft alone and in combination with platelet-rich plasma: a randomized clinical trial. J Periodontol 2004; 75: 1668-1677
  • 6 Kassolis JD, Reynolds MA. Evaluation of the adjunctive benefits of platelet-rich plasma in subantral sinus augmentation. J Craniofac Surg 2005; 16: 280-287
  • 7 Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 2002; 60: 1176-1181
  • 8 Jakse N, Tangl S, Gilli R, Berghold A, Lorenzoni M, Eskici A, et al. Influence of PRP on autogenous sinus grafts. An experimental study on sheep. Clin Oral Implants Res 2003; 14: 578-583
  • 9 Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH. Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg 2004; 33: 56-59
  • 10 Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der, Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor?. Clin Oral Implants Res 2005; 16: 349-356
  • 11 Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006; 24: 227-234
  • 12 Weibrich G, Kleis WK, Hitzler WE, Hafner G. Comparison of the platelet concentrate collection system with the plasma rich in growth factors kit to produce platelet-rich plasma: a technical report. Int J Oral Maxillofac Implants 2005; 20: 118-123
  • 13 Sánchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review. Int J Oral Maxillofac Implants 2003; 18: 93-103
  • 14 Whitman DH, Berry RL, Green DM. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997; 55: 1294-1299
  • 15 Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol 1998; 85: 638-646
  • 16 Tozum TF, Demiralp B. Platelet-rich plasma: a promising innovation in dentistry. J Can Dent Assoc 2003; 69: 664-9
  • 17 Weibrich G, Kleis WK, Hafner G, Hitzler WE, Wagner W. Comparison of platelet, leukocyte, and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank. Clin Oral Implants Res 2003; 14: 357-362
  • 18 Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 2000; 58: 297-300
  • 19 Sonnleitner D, Huemer P, Sullivan DY. A simplified technique for producing Platelet-rich plasma and Platelet Concentrate for intraoral bone grafting techniques: A technical note. Int J Oral Maxillofac Implants 2000; 15: 879-882
  • 20 Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002; 30: 97-102
  • 21 Tamimi FM, Montalvo S, Tresguerres I, Blanco LJerez. A comparative study of 2 methods for obtaining platelet-rich plasma. J Oral Maxillofac Surg 2007; 65: 1084-1093
  • 22 Anitua E. La utilización de los factores de crecimiento plasmáticos en cirugía oral, maxilofacial y periodoncia(PRGF). Rev Actual Odontoestomatol 2001; 6: 305-315
  • 23 Eby EW. Platelet-rich plasma: Harvesting with a singlespin centrifuge. J Oral Implantol 2002; 28: 297-301
  • 24 Weibrich G, Kleis WK. Curasan PRP kit vs. PCCS PRP system. Collection efficiency and platelet counts of two different methods for the preparation of platelet-rich plasma. Clin Oral Implants Res 2002; 13: 437-443
  • 25 Weibrich G, Hansen T, Kleis W, Buch R, Hitzler WE. Effect of platelet concentration in platelet-rich plasma on periimplant bone regeneration. Bone 2004; 34: 665-671
  • 26 Grageda E. Platelet-rich plasma and bone graft materials: a review and a standardized research protocol. Implant Dent 2004; 13: 301-309
  • 27 Messora MR, Nagata MJH, Mariano RC, Dornelles RC, Bomfim SR, Fucini SE. et al Bone healing in critical-size defects treated with platelet-rich plasma. A histologic and histometric study in rat calvaria. J Periodontal Res 2008; 43: 217-223
  • 28 Kim SG, Kim WK, Park JC, Kim HJ. A comparative study of osseointegration of Avana implants in a demineralized freeze-dried bone alone or with platelet-rich plasma. J Oral Maxillofac Surg 2002; 60: 1018-1025
  • 29 Efeoglu C, Akcay YD, Erturk S. A modified method for preparing platelet-rich plasma: an experimental study. J Oral Maxillofac Surg 2004; 62: 1403-1407
  • 30 Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP?. Implant Dent 2001; 10: 225-228
  • 31 Dugrillon A, Eichler H, Kern S, Kluter H. Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration. Int J Oral Maxillofac Surg 2002; 31: 615-619
  • 32 Schmitz G, Rothe G, Ruf A, Barlage S, Tschöpe D, Clemetson KJ. et al European Working Group on Clinical Cell Analysis: Consensus protocol for the flow cytometric characterization of platelet function. Thromb Haemost 1998; 79: 885-896
  • 33 Michelson AD. Flow cytometry: A clinical test of platelet function. Blood 1996; 87: 4925-4936
  • 34 Halmay D, Sótonyi P, Vajdovich P, Gaál T. Morphological evaluation of canine platelets on Giemsa- and PAS-stained blood smears. Acta Vet Hung 2005; 53: 337-350
  • 35 Hatakeyama M, Beletti ME, Zanetta-Barbosa D, Dechichi P. Radiographic and histomorphometric analysis of bone healing using autogenous graft associated with plateletrich plasma obtained by 2 different methods. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 105: e13-e18
  • 36 Kunz D. Possibilities and limitations of automated platelet counting procedures in the thrombocytopenic range. Semin Thromb Hemost 2001; 27: 229-235
  • 37 Langianni U, Limberti A, Bottari G, Ignesti C, Innocenti V. Evaluation of interferences in electronic platelet count. Quad Sclavo Diagn 1988; 24: 197-202
  • 38 Penev M, Kamenov V, Donkova O, Petkova D. Automatic and manual methods for counting the thrombocytes in the blood. Vutr Boles 1987; 26: 109-112
  • 39 Sutor AH, Grohmann A, Kaufmehl K, Wundisch T. Problems with platelet counting in thrombocytopenia. A rapid manual method to measure low platelet counts. Semin Thromb Hemost 2001; 27: 237-243
  • 40 Woodell-May JE, Ridderman DN, Swift MJ, Higgins J. Producing accurate platelet counts for platelet rich plasma: validation of a hematology analyzer and preparation techniques for counting. J Craniofac Surg 2005; 16: 749-759