Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin

Maiara Santos Gonçalves; Aron Ferreira da Silveira; Adriana de Andrade Batista Murashima; Maria Rossato; Miguel Angelo Hippolito

doi:10.1055/s-0039-1698782

International Archives of Otorhinolaryngology, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2020; 24(01): e47-e52
DOI: 10.1055/s-0039-1698782

Original Research

Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin

Maiara Santos Gonçalves

¹Department of Phonoaudiology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

,

Aron Ferreira da Silveira

²Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

,

Adriana de Andrade Batista Murashima

³Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil

,

Maria Rossato

³Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil

,

Miguel Angelo Hippolito

³Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirao Preto, SP, Brazil

› Institutsangaben

Abstract

Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover.

Objective To investigate the mechanisms involved in otoprotection by N-acetylcysteine through the expression of glutathione peroxidase in outer hair cells from rats treated with cisplatin.

Methods Male Wistar rats were intraperitoneally injected with cisplatin (8 mg/Kg) and/or received oral administration by gavage of N-acetylcysteine (300 mg/Kg) for 3 consecutive days. On the 4^th day, the animals were euthanized and beheaded. The tympanic bullae were removed and prepared for scanning electron microscopy and immunofluorescence.

Results Among the groups exposed to ototoxic doses of cisplatin, there was an increase in glutathione peroxidase immunostaining in two groups, the one exposed to cisplatin alone, and the group exposed to both cisplatin and N-acetylcysteine.

Conclusion The expression of glutathione peroxidase in the outer hair cells of rats exposed to cisplatin showed the synthesis of this enzyme under cellular toxicity conditions.

Keywords

cisplatin - toxicity - free radicals - cochlea - oxidative stress

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Referenzen

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