Osteologie 2019; 28(04): 294
DOI: 10.1055/s-0039-1700646
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Ex vivo analysis of microstructural bone parameters in tibia and femur in diabetes mellitus using high-resolution peripheral quantitative computed tomography

EM Wölfel
1   Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
,
AK Siebels
1   Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
2   Hamburg University of Technology, Hamburg, Germany
,
H Mushumba
3   Department of Forensic Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
,
B Wulff
3   Department of Forensic Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
,
K Püschel
3   Department of Forensic Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
,
M Amling
1   Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
,
C Glüer
4   Department of biomedical imaging, Clinic for Raiology and Neuroradiology, University Medical Center Schleswig-Holstein, Kiel, Germany
,
B Busse
1   Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
14 November 2019 (online)

Also available at
 

Introduction:

Individuals with type 2 diabetes mellitus (T2DM) have a higher fracture risk despite presenting with normal or high bone mineral density (BMD). Hence, differences in bone microstructure or bone quality might lead to an increased fracture risk.

Material and Methods:

A clinical high-resolution peripheral quantitative computed tomography (HR-pQCT) system is used to analyze human cortical bone samples ex vivo to determine geometry and structure parameters of the bone. Currently 37 human bone samples of the mid-diaphysis of tibia and femur from male organ donors were analyzed following autopsy. A resolution of 82 µm was applied to determine the bone structure parameters: cortical porosity (Ct.Po), cortical volumetric bone mineral density (Ct.vBMD) and cortical thickness (Ct.Th).

Results:

Based on age and diabetes diagnosis, samples were divided into three groups: i) ''T2DM'' with 60 – 90 years ii) age-matched ''Controls'' and iii) ''5th/6th decade'' with 40 – 59 years. The individuals of the T2DM and Control group are divided into two subgroups based on their level of cortical porosity measured with HR-pQCT. After splitting the groups, the high porosity T2DM group showed lower Ct.vBMD in the tibia and femur compared to 5th/6th decade and low porosity groups. Also significantly lower Ct.vBMD was found in the high porosity Control group with regard to 5th/6th decade and low porosity T2DM group only in the tibia. Ct.Th was similar between all groups.

Discussion:

Our analysis show that Ct.vBMD is lower in individuals with high cortical porosity in the tibia and femur and confirm the existence of a high porosity sub-group in T2DM.