Application of Cationic Conjugated Polymer–Outer Membrane Vesicle Complexes in Inhibiting Red Blood Cell Aggregation

Abstract

Cationic conjugated polymers (CCPs) have been attracting a lot of attention in biomedical applications because of their good photoelectric properties and good cell viability. However, positively charged components may reduce the electrostatic repulsion among red blood cells (RBCs) and induce RBC aggregation, which may lead to thrombus. Herein, in this work, we prepared the complexes of CCPs and outer membrane vesicles (OMVs) to inhibit RBC aggregation induced by CCPs and improve the biocompatibility of CCPs. A poly(fluorene-co-phenylene) derivative (PFP), a poly(p-phenylenevinylene) derivative (PPV), and a poly(thiophene) derivative (PMNT) were chosen as the model CCPs, and OMVs were used as the representative of a cell membrane. The CCP–OMV complexes were formed mainly driven by electrostatic interactions. Besides, the electrostatic CCP–OMV complexes were proved to be able to prevent the RBC aggregation induced by CCPs while maintaining the hydrophobic interactions between CCPs and RBCs.

Key words

cationic conjugated polymers - outer membrane vesicles - inhibition of RBC aggregation

Supporting Information

Supporting information for this article is available online at https://doi.org/10.1055/s-0039-1700849.

• References and Notes

• 1 Wang TT, Jiang X. ACS Appl. Mater. Interfaces 2015; 7: 129
2
• 2a Han Y, Wang X, Dai H, Li S. ACS Appl. Mater. Interfaces 2012; 4: 4616
• 2b Jan KM, Chien S. J. Gen. Physiol. 1973; 61: 638
• 2c Jan KM. Bioelectrochemistry 1980; 188: 143
• 2d Snabre P, Grossmann GH, Mills P. Colloid Polym. Sci. 1985; 263: 478
• 2e Armstrong JK, Meiselman HJ, Fisher TC. Am. J. Hematol. 1997; 56: 26
• 2f Toth K, Wenby RB, Meiselman HJ. Biorheology 2000; 37: 301
• 2g Byrnes JR, Wolberg AS. Blood 2017; 130: 1795
• 3 Zhu CL, Liu LB, Yang Q, Lv FT, Wang S. Chem. Rev. 2012; 112: 4687
4
• 4a Disney MD, Zheng J, Swager TM, Seeberger PH. J. Am. Chem. Soc. 2004; 126: 13343
• 4b Yuan HX, Liu Z, Liu LB, Lv FT, Wang YL, Wang S. Adv. Mater. 2014; 26: 4333
• 4c Zhu CL, Yang Q, Liu LB. , et al. Adv. Mater. 2011; 23: 4805
5
• 5a Li M, Li SL, Chen H, Hu R, Liu LB, Lv FT, Wang S. ACS Appl. Mater. Interfaces 2016; 8: 42
• 5b Li M, He P, Li SL. , et al. ACS Biomater. Sci. Eng. 2018; 4: 2037
6
• 6a Duan XR, Li ZP, He F, Wang S. J. Am. Chem. Soc. 2007; 129: 4154
• 6b Xia F, Zuo XL, Yang RQ. , et al. J. Am. Chem. Soc. 2010; 132: 1252
7
• 7a Zhu CL, Yang Q, Liu LB, Wang S. Chem. Commun. (Camb.) 2011; 47: 5524
• 7b Wang B, Nie CY, Liu LB, Lv FT, Wang S. Chin. Chem. Lett. 2017; 28: 1975
8
• 8a Feng GG, Mai CK, Zhan RY, Bazan GC, Liu B. J. Mater. Chem. B Mater. Biol. Med. 2015; 3: 7340
• 8b Huang Y, Pappas HC, Zhang LQ. , et al. Chem. Mater. 2017; 29: 6389
9
• 9a Dehaini D, Fang RH, Zhang LF. Bioeng. Transl. Med. 2016; 1: 30
• 9b Kroll AV, Fang RH, Zhang LF. Bioconjug. Chem. 2017; 28: 23
• 9c Su JH, Sun HP, Meng QS. , et al. Adv. Funct. Mater. 2016; 26: 1243
• 9d Gao WW, Fang RH, Thamphiwatana S. , et al. Nano Lett. 2015; 15: 1403
• 9e Hu CMJ, Fang RH, Copp J, Luk BT, Zhang LF. Nat. Nanotechnol. 2013; 8: 336
• 9f Yang G, Liu L, Lv F, Wang S. Sci. Rep. 2013; 3: 1702
• 10 Gaylord BS, Massie MR, Feinstein SC, Bazan GC. Proc. Natl. Acad. Sci. U.S.A. 2005; 102: 34
• 11 Yuan HX, Liu Z, Liu LB, Lv FT, Wang YL, Wang S. Adv. Mater. 2014; 26: 4333-4338
• 12 Liu XF, Tang YL, Wang LH, Zhang J, Song SP, Fan CH, Wang S. Adv. Mater. 2007; 19: 1471-1474
• 13 The preparation of CCP-OMVs complex: PPV storage solution (10 mM) was added into the OMVs solution (80 μg/mL) to obtain a final concentration of PPV in 10 μM or 100 μM. Then the mixture was placed in room temperature for 4 h to obtain PPV-OMVs. PFP-OMVs and PMNT-OMVs were prepared by the same way as PPV-OMVs