Anthropometric and Metabolic Responses in FTO rs9939609 Gene Polymorphism after a Multidisciplinary Lifestyle Intervention in Overweight and Obese Adolescents

Cláudia Daniela Barbian; Cézane Priscila Reuter; Jane Dagmar Pollo Renner; Priscila Tatiana da Silva; Elisa Inês Klinger; James Philip Hobkirk; Andréia Rosane de Moura Valim; Miria Suzana Burgos

doi:10.1055/s-0039-3399511

Journal of Pediatric Genetics, Table of Contents

J Pediatr Genet 2020; 09(01): 019-026
DOI: 10.1055/s-0039-3399511

Original Article

Georg Thieme Verlag KG Stuttgart · New York

Anthropometric and Metabolic Responses in FTO rs9939609 Gene Polymorphism after a Multidisciplinary Lifestyle Intervention in Overweight and Obese Adolescents

Cláudia Daniela Barbian

¹Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

Cézane Priscila Reuter

²Department of Physical Education and Health, Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

Jane Dagmar Pollo Renner

³Department of Biology and Pharmacy, Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

Priscila Tatiana da Silva

¹Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

Elisa Inês Klinger

¹Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

James Philip Hobkirk

⁴Department of Sport, Health and Exercise Sciences, Faculty of Health Sciences, School of Life Sciences, University of Hull, Hull, United Kingdom

,

Andréia Rosane de Moura Valim

³Department of Biology and Pharmacy, Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

,

Miria Suzana Burgos

²Department of Physical Education and Health, Graduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, Rio Grande do Sul, Brazil

› Author Affiliations

Abstract

Few studies show the potential changing effect of fat-mass and obesity-associated (FTO) rs9939609 gene on cardiometabolic risk after a lifestyle intervention. This study aims to evaluate whether overweight and obese adolescents, carriers of the risk genotypes for obesity of the FTO rs9939609 gene polymorphism, have different anthropometric and biochemical responses to an interdisciplinary intervention program. The quasi-experimental study involved 34 adolescents aged 10 to 15 years. Schoolchildren with AA/AT genotype decreased glucose, total cholesterol, low-density lipoprotein cholesterol, and increased high-density lipoprotein cholesterol. However, there were no differences between the genotypes, suggesting that the “A” allele did not modify the subject's response to the intervention program.

Keywords

clinical trial - obesity - genetic polymorphism

Full Text

References

References
1 Ogden CL, Carroll MD, Flegal KM. High body mass index for age among US children and adolescents, 2003-2006. JAMA 2008; 299 (20) 2401-2405
2 Wang Y, Lobstein T. Worldwide trends in childhood overweight and obesity. Int J Pediatr Obes 2006; 1 (01) 11-25
3 Cárdenas Fuentes G, Bawaked RA, Martínez González MÁ. , et al. Association of physical activity with body mass index, waist circumference and incidence of obesity in older adults. Eur J Public Health 2018; 28 (05) 944-950
4 Schröder H, Mendez MA, Ribas L. , et al. Caloric beverage drinking patterns are differentially associated with diet quality and adiposity among Spanish girls and boys. Eur J Pediatr 2014; 173 (09) 1169-1177
5 Luczyński W, Fendler W, Ramatowska A. , et al. Polymorphism of the FTO gene influences body weight in children with type 1 diabetes without severe obesity. Int J Endocrinol 2014; 2014: 630712
6 Zhang M, Zhao X, Xi B. , et al. Impact of obesity-related gene polymorphism on risk of obesity and metabolic disorder in childhood [in Chinese]. Zhonghua Yu Fang Yi Xue Za Zhi 2014; 48 (09) 776-783
7 Quan LL, Wang H, Tian Y, Mu X, Zhang Y, Tao K. Association of fat-mass and obesity-associated gene FTO rs9939609 polymorphism with the risk of obesity among children and adolescents: a meta-analysis. Eur Rev Med Pharmacol Sci 2015; 19 (04) 614-623
8 Yang M, Xu Y, Liang L. , et al. The effects of genetic variation in FTO rs9939609 on obesity and dietary preferences in Chinese Han children and adolescents. PLoS One 2014; 9 (08) e104574
9 Fredriksson R, Hägglund M, Olszewski PK. , et al. The obesity gene, FTO, is of ancient origin, up-regulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology 2008; 149 (05) 2062-2071
10 Gerken T, Girard CA, Tung YC. , et al. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase. Science 2007; 318 (5855): 1469-1472
11 Tanofsky-Kraff M, Han JC, Anandalingam K. , et al. The FTO gene rs9939609 obesity-risk allele and loss of control over eating. Am J Clin Nutr 2009; 90 (06) 1483-1488
12 Qi Q, Downer MK, Kilpeläinen TO. , et al. Dietary intake, FTO genetic variants, and adiposity: a combined analysis of over 16,000 children and adolescents. Diabetes 2015; 64 (07) 2467-2476
13 Andreasen CH, Stender-Petersen KL, Mogensen MS. , et al. Low physical activity accentuates the effect of the FTO rs9939609 polymorphism on body fat accumulation. Diabetes 2008; 57 (01) 95-101
14 World Health Organization. Young People's Health – a Challenge for Society. Report of a WHO Study Group on Young People and Health for All. Technical Report Series 731. Geneva: WHO; 1986
15 Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 2007; 39 (02) 175-191
16 Hulley SB, Cummings SR, Browner WS. , et al. Designing Clinical Research: An Epidemiologic Approach, 4th ed. Philadelphia, PA: Lippincott Williams & Wilkins; 2013
17 World Health Organization. Growth reference 5–19 years. 2007 . Available at: http://www.who.int/growthref/who2007_bmi_for_age/en/ . Accessed December 10, 2018
18 Fernández JR, Redden DT, Pietrobelli A, Allison DB. Waist circumference percentiles in nationally representative samples of African-American, European-American, and Mexican-American children and adolescents. J Pediatr 2004; 145 (04) 439-444
19 Picon PX, Leitao CB, Gerchman F. , et al. Waist measure and waist-to-hip ratio and identification of clinical conditions of cardiovascular risk: multicentric study in type 2 diabetes mellitus patients. Arq Bras Endocrinol Metab 2007; 51 (03) 443-449
20 Slaughter MH, Lohman TG, Boileau RA. , et al. Skinfold equations for estimation of body fatness in children and youth. Hum Biol 1988; 60 (05) 709-723
21 Lohman TG. The use of skinfold to estimate body fatness on children and youth. JOPERD 1987; 58 (09) 98-102
22 Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28 (07) 412-419
23 Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988; 16 (03) 1215
24 Sociedade Brasileira de Cardiologia; Sociedade Brasileira de Nefrologia; Sociedade Brasileira de Hipertensão. VI Brazilian Guidelines on Hypertension. Arq Bras Cardiol 2010; 95 (01) (Suppl. 01) 1-51
25 Tanner JM. Growth at Adolescence, 2nd.ed. Oxford: Blackwell Scientific; 1962
26 ABEP. Brazilian Market Research Association. Brazilian Criteria 2016. Available at: http://www.abep.org/criterio-brasil . Accessed December 10, 2018
27 Sullivan GM, Feinn R. Using effect size-or why the P value is not enough. J Grad Med Educ 2012; 4 (03) 279-282
28 Moraes G, Reuter CP, Renner JDP. , et al. Genotypic carriers of the obesity-associated FTO polymorphism exhibit different cardiometabolic profiles after an intervention. An Acad Bras Ciênc 2016; 88 (04) 2331-2339
29 do Nascimento GA, Teixeira MD, Furtado-Allee L. , et al. FTO rs9939609 A allele influences anthropometric outcome in response to dietary intervention, but not in response to physical exercise program. Eur J Nutr 2019; 58 (01) 325-334
30 Müller TD, Hinney A, Scherag A. , et al. ‘Fat mass and obesity associated’ gene (FTO): no significant association of variant rs9939609 with weight loss in a lifestyle intervention and lipid metabolism markers in German obese children and adolescents. BMC Med Genet 2008; 9: 85
31 Lappalainen TJ, Tolppanen AM, Kolehmainen M. , et al; Finnish Diabetes Prevention Study Group. The common variant in the FTO gene did not modify the effect of lifestyle changes on body weight: the Finnish Diabetes Prevention Study. Obesity (Silver Spring) 2009; 17 (04) 832-836
32 Livingstone KM, Celis-Morales C, Papandonatos GD. , et al. FTO genotype and weight loss: systematic review and meta-analysis of 9563 individual participant data from eight randomised controlled trials. BMJ 2016; 354: i4707
33 Zou ZC, , -J Mao L, Shi YY, Chen JH, Wang LS, Cai W. Effect of exercise combined with dietary intervention on obese children and adolescents associated with the FTO rs9939609 polymorphism. Eur Rev Med Pharmacol Sci 2015; 19 (23) 4569-4575
34 Rendo T, Moleres A, Marti Del Moral A. Effects of the FTO gene on lifestyle intervention studies in children. Obes Facts 2009; 2 (06) 393-399
35 Leońska-Duniec A, Ahmetov II, Zmijewski P. Genetic variants influencing effectiveness of exercise training programmes in obesity - an overview of human studies. Biol Sport 2016; 33 (03) 207-214
36 Xiang L, Wu H, Pan A. , et al. FTO genotype and weight loss in diet and lifestyle interventions: a systematic review and meta-analysis. Am J Clin Nutr
37 Celis-Morales C, Marsaux CF, Livingstone KM. , et al; Food4Me Study. Physical activity attenuates the effect of the FTO genotype on obesity traits in European adults: the Food4Me study. Obesity (Silver Spring) 2016; 24 (04) 962-969
38 Petkeviciene J, Smalinskiene A, Klumbiene J, Petkevicius V, Kriaucioniene V, Lesauskaite V. Physical activity, but not dietary intake, attenuates the effect of the FTO rs9939609 polymorphism on obesity and metabolic syndrome in Lithuanian adult population. Public Health 2016; 135: 23-29
39 Oyeyemi BF, Ologunde CA, Olaoye AB, Alamukii NA. FTO gene associates and interacts with obesity risk, physical activity, energy intake, and time spent sitting: pilot study in a Nigerian population. J Obes 2017; 2017: 3245270
40 Ruiz JR, Labayen I, Ortega FB. , et al; HELENA Study Group. Attenuation of the effect of the FTO rs9939609 polymorphism on total and central body fat by physical activity in adolescents: the HELENA study. Arch Pediatr Adolesc Med 2010; 164 (04) 328-333
41 Xi B, Shen Y, Zhang M. , et al. The common rs9939609 variant of the fat mass and obesity-associated gene is associated with obesity risk in children and adolescents of Beijing, China. BMC Med Genet 2010; 11: 107
42 Kilpeläinen TO, Qi L, Brage S. , et al. Physical activity attenuates the influence of FTO variants on obesity risk: a meta-analysis of 218,166 adults and 19,268 children. PLoS Med 2011; 8 (11) e1001116
43 Reuter CP, Rosane De Moura Valim A, Gaya AR. , et al. FTO polymorphism, cardiorespiratory fitness, and obesity in Brazilian youth. Am J Hum Biol 2016; 28 (03) 381-386
44 Burgess E, Hassmén P, Pumpa KL. Determinants of adherence to lifestyle intervention in adults with obesity: a systematic review. Clin Obes 2017; 7 (03) 123-135
45 Fidelix YL, Farias Júnior JC, Lofrano-Prado MC, Guerra RL, Cardel M, Prado WL. Multidisciplinary intervention in obese adolescents: predictors of dropout. Einstein (Sao Paulo) 2015; 13 (03) 388-394