Planta Med 2019; 85(18): 1390
DOI: 10.1055/s-0039-3399636
Pre-Congress Symposia
African Research Workshop
© Georg Thieme Verlag KG Stuttgart · New York

Tyrosinase TLC-autography for South African indigenous tea, Athrixia phylicoides DC, and potential use as a cosmeceutical extract

VS Thibane
1   University of South Africa, Department of Agriculture and Animal Health, Florida Science Campus, Roodepoort 1709, South Africa
,
ML Ramphinwa
1   University of South Africa, Department of Agriculture and Animal Health, Florida Science Campus, Roodepoort 1709, South Africa
2   University of Venda, Department of Horticultural Sciences, Thohoyandou 0950, South Africa
,
FN Mudau
1   University of South Africa, Department of Agriculture and Animal Health, Florida Science Campus, Roodepoort 1709, South Africa
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Athrixia phylicoides DC (bush tea), a South African indigenous tea, has been consumed in rural communities with its nutritional and medicinal attributes well documented [1]. The low consumption of bush tea in more urbanized areas means the tea can not immulate the success of other South African herbal teas such as rooibos and honeybush [2]. Therefore, the aim of the study was to assess plant metabolites of bush tea for their inhibition of tyrosinase using a TLC (thin layer chromatography)-based autography assay [3]. Ethanol extracts were spotted on TLC (silica gel 60 F245) plate and separated using eluent systems EMW [(Ethyl acetate: Methanol: Water, 40:5.4:4), CEF (Chloroform: Ethyl acetate: Formic acid, 5:4:1), BEA (Benzene: Ethanol: Ammonium hydroxide, 90:10:1)] and their inhibition of tyrosinase evaluated. The competitive and non-competitive inhibitory activity of active metabolites were further tested using a microtiter plate. Metabolites were identified by their retention factor (Rf) value. Gallic acid, chlorogenic acid and quercetin separated with the EMW eluent system expressed anti-tyrosinase activity similar to kojic acid. The prevalence of a dark ring on the TLC plate was indicative of melanin formation while a clear spot indicating inhibition of tyrosinase. Active metabolites with Rf values different from kojic acid were identified with the CEF eluent system. Furthermore, metabolites separated with the BEA eluent system were found not to inhibit tyrosinase. Active metabolites expressed a non-competitive behaviour with a Km equivalent to kojic acid. The findings from the study reported an alternative usage of bush tea as a natural cosmeceutical extract.

Refers to:

Abstract see SL AR-06Planta Med 2019; 85(18): P-493
DOI: 10.1055/s-0039-3400103

 

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