Planta Med 2019; 85(18): 1410
DOI: 10.1055/s-0039-3399691
Abstracts of Short Lectures
Short Lectures Monday, September 02, 2019
Short Lectures C: Biological and Pharmacological Activities of Natural Products
© Georg Thieme Verlag KG Stuttgart · New York

Root extracts from Ononis spinosa exert anti-inflammatory activity in vitro on IL-8 and TNF-α release by inhibition of TLR-4 receptor

V Spiegler
1   University of Münster, PharmaCampus,, Corrensstraße 48, D-48149 Münster, Germany
,
B Michalak
2   Medical University of Warsaw,, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw, Poland
,
J Addotey
3   Kwame Nkrumah University of Science and Technology,, Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Kumasi, Ghana
,
T Saenger
1   University of Münster, PharmaCampus,, Corrensstraße 48, D-48149 Münster, Germany
,
J Jose
1   University of Münster, PharmaCampus,, Corrensstraße 48, D-48149 Münster, Germany
,
AK Kiss
2   Medical University of Warsaw,, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw, Poland
,
A Hensel
1   University of Münster, PharmaCampus,, Corrensstraße 48, D-48149 Münster, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Extracts from the roots of Ononis spinosa L. are traditionally used for urinary tract infections and rheumatic conditions. A reduction of oedema in the carrageenan-induced rat paw oedema test suggested an anti-inflammatory activity in vivo [1]. Recent investigations indicated the isoflavone sativanone to be a potent inhibitor of human hyaluronidase-1, an enzyme strongly related to induction of inflammatory cellular response [2]. During further investigations, a dichloromethane extract fully characterized by LC-MS studies showed concentration-dependent inhibition of IL-8 and TNF-α release from LPS prestimulated human neutrophils. This inhibitory activity was shown to be mainly due to the presence of the norneolignan clitorienolactone B and the triterpene α-onocerin. In addition, extract as well as clitorienolactone B and α-onocerine significantly decreased the expression of adhesion molecules CD11b/CD18 and conversely increased the expression of CD62L in LPS-stimulated human neutrophils, a finding that is in line with reduced inflammatory response by inhibition of adhesion and migration of immune cells.

As all of the observed effects are potentially mediated via the Toll-like receptor 4 (TLR4) signaling pathway, TLR4 transfected HEK293 cells and non-transfected HEK293 cells as control were incubated with the dichloromethane extract. LPS-induced IL-8 secretion was significantly inhibited in a concentration-dependent manner, confirming TLR-4 antagonism. An aqueous extract from the roots of O. spinosa also showed antagonistic effects.

This study rationalizes the traditional use of extracts from O. spinosa for therapy of urinary tract infections and rheumatic conditions, due to its potential anti-inflammatory effects that are in part mediated via TLR-4 receptor antagonism.

 

  • References

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