Subscribe to RSS
DOI: 10.1055/s-0039-3399739
The in vitro antibacterial activity and safety of Morinda lucida leaf extracts against Salmonella serovars relevant in livestock infections
Publication History
Publication Date:
20 December 2019 (online)
Salmonella infections are of great importance in human and animal health. Infections caused by non-typhoidal Salmonella are either non-invasive or invasive systemic infections that require effective antimicrobial therapy. The aim of this study was to investigate the antibacterial activity, safety and anti-biofilm potential of leaf extracts of Morinda lucida against eight Salmonella serovars. M. lucida is used in traditional treatment of human typhoid and malaria fever. Acetone and aqueous leaf extracts of M. lucida were screened for antibacterial activity against several serovars of Salmonella enterica subsp. enterica including S. enterica serovar Gallinarum (birds), Dublin (birds and ruminants), Choleraesuis (pigs), Braenderup (birds), Idikan (humans and birds), Kottbus (birds), Typhimurium (birds and ruminants) and Enteritidis (birds and humans) using a serial microdilution assay. The cytotoxic and anti-biofilm potential of the acetone and aqueous extracts were also determined against human colon cancer (Caco-2) cells and Salmonella biofilm formation respectively.
The minimum inhibitory concentration (MIC) of the extracts ranged from 0.09 to 1.87 mg/ml. The LC50 values of the acetone and aqueous extracts against the Caco-2 cells were 0.46 and 0.33 mg/ml respectively. The acetone extract had the strongest anti-biofilm activity against S. Enteritidis. The range of selectivity index (SI) values of the acetone and aqueous extracts was 1.00 to 6.57 and 0.23 to 8.28 respectively. The potential usefulness of this plant species as an alternative for treatment of human and animal salmonellosis supported by these results. However, in vivo data is necessary to further investigate this claim.
-
References
- 1 Davies R. Salmonellosis. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals OIE, World Organization for Animal Health. 2004: 1018-1032.
- 2 Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998; 64: 711-713.