Planta Med 2019; 85(18): 1437
DOI: 10.1055/s-0039-3399762
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Matrix free laser desorption ionization as a versatile tool for the chemical characterization of complex mixtures of phenolic compounds

A Schinkovitz
1   University of Angers, SONAS/SFR QUASAV,, Campus du Végétal, 42 rue Georges Morel, 49070 Beaucouzé, France
,
S Boisard
1   University of Angers, SONAS/SFR QUASAV,, Campus du Végétal, 42 rue Georges Morel, 49070 Beaucouzé, France
,
M Škopíková
1   University of Angers, SONAS/SFR QUASAV,, Campus du Végétal, 42 rue Georges Morel, 49070 Beaucouzé, France
,
M Gaucher
2   INRA, UMR1345 IRHS,, 42 rue Georges Morel 49071 Beaucouzé, France
,
I Freuze
3   University of Angers,, SFR MATRIX, 2 bd. Lavoisier, 49045, Angers, France
,
J Osuga
4   JEOL Europe SAS,, 1 allée de Giverny, 78290 Croissy Sur Seine, France
5   Open Innovation Joint Research Laboratory Project Research Center for Fundamantal Science, Graduate School of Science, Osaka University,, 1-1 Machikaneyama, Toyonaka, 560-0043, Osaka, Japan
,
M Hashimoto
4   JEOL Europe SAS,, 1 allée de Giverny, 78290 Croissy Sur Seine, France
,
MN Brisset
2   INRA, UMR1345 IRHS,, 42 rue Georges Morel 49071 Beaucouzé, France
,
P Richomme
1   University of Angers, SONAS/SFR QUASAV,, Campus du Végétal, 42 rue Georges Morel, 49070 Beaucouzé, France
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Publikationsdatum:
20. Dezember 2019 (online)

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The rapid and precise chemical characterization of complex mixtures of small molecules is essential for contemporary natural products’ (Nps) research, as well as for the quality control of herbal medicines and dietary supplements. While LC-MS is generally considered as method of first choice to meet these analytical challenges, the current work will present matrix free laser desorption ionization (LDI) as a versatile supplement or potential alternative to this approach [1], [2].

Among others, particularly phenolic NPs exhibit close structural similarities to matrices used in matrix assisted laser desorption ionization. Consequently, these compounds may be ionized by laser irradiation without matrix support. This concept is being outlined by two examples:

The first focuses on the chemical profiling of propolis obtained from different global regions, where high resolution LDI-MS permitted the identification of 34 compounds (mostly cinnamic acid and flavonoid derivatives) from a crude extract of French propolis. A contemporaneously performed LC-ESI-MS experiment solely detected 23 of these compounds. Moreover, LDI-MS facilitated the differentiation of propolis samples according to their origin by principal compound analysis (PCA). The second example discusses the LDI-monitoring of the metabolic profiles of apple seedlings (Malus x domestica) after being treated with various elicitors. Selected plants were further infected by Erwinia amylovora, the causative agent of fire blight. Again LDI-MS permitted the detection of key-metabolites (e.g. caffeic acid, phloridzin, and quercitrine) and also facilitated PCA grouping according to the applied treatment.

These results highlight the interesting prospective of LDI-MS as powerful analytical tool and supplement to existing methods.

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Fig. 1
 

  • References

  • 1 Schinkovitz A, Boisard S, Freuze I, Osuga J, Mehlmer N, Brück T, Richomme P. Matrix-free laser desorption ionization mass spectrometry as a functional tool for the analysis and differentiation of complex phenolic mixtures in propolis: a new approach to quality control. Anal Bioanal Chem 2018; 410: 6187-6195.
    CrossrefPubMedGoogle Scholar
  • 2 LePogam P, Schinkovitz A, Legouin B, Le Lamer AC, Boustie J, Richomme P. Matrix-free UV-laser desorption ionization mass spectrometry as a versatile approach for accelerating dereplication studies on lichens. Anal Chem 2015; 87: 10421-10428.
    CrossrefPubMedGoogle Scholar