Planta Med 2019; 85(18): 1444
DOI: 10.1055/s-0039-3399783
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Induction of secondary metabolism of marine derived Streptomyces cacaoi

E Gezer
1   Department of Biotechnology and Bioengineering, Izmir Institute of Technology,, 35430, Urla, Izmir, Turkey
,
E Bilgi
2   Department of Bioengineering, Faculty of Engineering, Izmir Institute of Technology,, 35430, Urla, Izmir, Turkey
,
M Küçüksolak
2   Department of Bioengineering, Faculty of Engineering, Izmir Institute of Technology,, 35430, Urla, Izmir, Turkey
,
E Bedir
2   Department of Bioengineering, Faculty of Engineering, Izmir Institute of Technology,, 35430, Urla, Izmir, Turkey
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

Also available at
 

Microbial natural products have an adaptive role as signal molecules or defense tools in ecological interactions [1]. Biosynthesis of these molecules is suppressed in standard laboratory conditions where there are no ecological triggers. Thus, only a portion of the chemical diversity of a microbial strain is discovered by standard fermentation protocols. However, using different fermentation conditions or different approaches such as co-culture, biosynthesis of these suppressed molecules can be triggered, and new natural products can be isolated [2],[3].

In our previous studies, it was demonstrated that marine derived Streptomyces cacaoi had a potent antimicrobial effect against Enterecoccus feacium and MRSA [4]. A comprehensive statistical optimization of this promising strain was aimed to enrich the chemical diversity of the ethyl acetate extract and to increase its bioactivity. Response surface methodology was used for statistical optimization of some fermentation paramaters. As responses, the chemical diversities of the ethyl acetate extracts were monitored by HPLC-DAD system, and the antimicrobial effects were determined by disc diffusion assay on Bacillus subtilis. As a result, the diameter of the inhibition zones (150 µg extract) varied between 6 to 25.5 mm against Bacillus subtilis. Also, chemical profiles of the extracts varied even in the major compounds. Consequently, this study demonstrated the importance of optimization protocols for the discovey of new/novel molecules in microbial natural product research.

 

  • References

  • 1 Traxler MF, Kolter R. Natural products in soil microbe interactions and evolution. Nat Prod Rep 2015; 32: 956-970
    CrossrefPubMedGoogle Scholar
  • 2 Abdelmohsen UR, Grkovic T, Balasubramanian S, Kamel MS, Quinn RJ, Hentschel U. Elicitation of secondary metabolism in actinomycetes. Biotechnol Adv 2015; 1 (33) (6 Pt 1): 798-811
    PubMedGoogle Scholar
  • 3 Bibb MJ. Regulation of secondary metabolism in streptomycetes. Curr Opin Microbiol 2005; 8: 208-215
    CrossrefPubMedGoogle Scholar
  • 4 Khan N, Yılmaz S, Aksoy S, Uzel A, Tosun Ç, Ballar Kirmizibayrak P, Bedir E. Polyethers isolated from the marine actinobacterium Streptomyces cacaoi inhibit autophagy and induce apoptosis in cancer cells. Chem Biol Interact 2019; 307: 167-178
    CrossrefPubMedGoogle Scholar