Planta Med 2019; 85(18): 1449-0
DOI: 10.1055/s-0039-3399795
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

How light photoperiod and medium composition could increase the production of a potent anticancer metabolite by Nostoc

A Polyzois
1   Laboratoire des Produits naturels, Analyse et Synthèse, CITCOM -Cibles Thérapeutiques et Conception de Médicaments, UMR 8038 CNRS- Faculté de Pharmacie de Paris, Université Paris Descartes,, 4 Avenue de l’Observatoire, 75006 Paris, France
2   Institute for Integrative Biology of the Cell, Commissariat à l’Energie Atomique, CNRS, Université Paris-Sud, Université Paris-Saclay,, 91198 Gif sur Yvette, France
,
S Michel
1   Laboratoire des Produits naturels, Analyse et Synthèse, CITCOM -Cibles Thérapeutiques et Conception de Médicaments, UMR 8038 CNRS- Faculté de Pharmacie de Paris, Université Paris Descartes,, 4 Avenue de l’Observatoire, 75006 Paris, France
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Cryptophycin-1 is the first circular depsipeptide which was isolated in 1990 from Nostoc sp. as a tubulin-inhibitor with potential anticancer activity. The total synthesis of cruptophycin faces stereoselctivity issues and its yield is only moderate (<13%), and unsuitable for the industrial production [1].

These considerations provide a rationale to investigate alternative solutions, like stressing the environmental conditions of the culture (ATCC53789) in order to find the optimal for the metabolite’s over-production (more than 0.52mg/L of culture) [2]. Here, we examine the effect of light photoperiod and media’s composition. In light photoperiod we tested 24:0,16:8,12:12,8:16 (Light:Dark). In medium composition, Nitrogen’s concentration was examined to test the implication of heterocysts, a N-fixing cell type. The culture was cultivated in a light-box, the biomass of a 20ml-sample was extracted and then metabolite’s concentration was calculated through HPLC. Lastly, the “growth/time” and “metabolite’s concentration/time” curves were drawn.

Remarkably, photoperiod has an opposite effect on the two curves. Regarding the growth, the optimal period was 16:8>12:12>8:16>24:0 (Light:Dark), however the inverse happens for the cryptophycin’s production. It is shown that even if the presence of non-light is needed for the cell’s growth, the metabolite’s production is higher under constant light conditions.

Regarding the Nitrogen concentration, and so the implication of heterocysts, it was noticed that they were not involved in the depsipeptide’s expression process.

These stress-tests’ results contribute on the formation of the optimal culture conditions for the metabolite’s overproduction. That would progress the research and development of the novel potential anticancer drug.