Planta Med 2019; 85(18): 1454
DOI: 10.1055/s-0039-3399809
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Liposomal incorporation of Thymus essential oils and in vitro antibacterial activity

M Kindl
1   Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb,, Trg Marka Marulića 20, Zagreb, Croatia
,
Ž Vanić
2   Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Zagreb,, Domagojeva 2, Zagreb, Croatia
,
HČ Paljetak
3   Center for Translational and Clinical Research, School of Medicine, University of Zagreb,, Šalata 2, Zagreb, Croatia
,
D Verbanac
4   Faculty of Pharmacy and Biochemistry, University of Zagreb,, Department of Medical Biochemistry and Haematology, Domagojeva 2, Zagreb, Croatia
,
F Bucar
5   Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4/I, Graz, Austria
,
S Vladimir-Knežević
1   Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb,, Trg Marka Marulića 20, Zagreb, Croatia
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Publikationsverlauf

Publikationsdatum:
20. Dezember 2019 (online)

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Essential oils (EOs) of Thymus species have been known to possess antimicrobial properties and therefore are of great interest in pharmaceutical, food and cosmetic industry. However, they are poorly soluble in water, unstable and susceptible to degradation. Encapsulation of EOs in different nanocarriers represents a valid strategy to overcome these limitations as well as to enhance their biological activity [1], [2]. The present study aimed to evaluate the antibacterial potential of liposomally-encapsulated phenol rich EOs from T. longicaulis C. Presl, T. pulegioides L. and T. vulgaris L. originating from Croatia. The commercial T. vulgaris EO was used for the optimization of liposomes’ composition and preparation procedure. Based on the high encapsulation of EO and satisfied physical properties achieved, liposomes containing 20 mg/mL of soy phosphatidylcholine, 2 mg/mL of cholesterol and 5 mg/mL of EO were selected as optimal for testing the antibacterial activities against S. aureus, E. faecalis and E. coli. The mean diameters of liposomes were 187–216 nm with polydispersity indexes from 0.43 to 0.53, while encapsulation efficiency varied between 51% and 57%. The obtained minimal inhibitory concentrations (MICs) were in the range 0.25–2 mg/mL. Encapsulation of the Thymus EOs in liposomes improved their solubility, stability and enhanced their antibacterial activities exhibiting MICs two-fold lower than the MICs of the corresponding pure EOs against S. aureus and E. coli. Liposomally-encapsulated T. longicaulis EO demonstrated the strongest antibacterial effect showing four-fold increase of activity against E. faecalis, thus indicating its potential use as biopreservative and natural remedy.