Study of disturbed metabolism correction in tumor cells treated by combination of iron oxide nanoparticles and Limonium extract

It is known that metabolic pathways are tightly connected with key signs of malignant tumor: heterogeneity, metastasis, reprogramming of the cellular environment. The aim of the work was to study the correction of metabolic disorders of the experimental tumor (sarcoma 45) with the help of the extract from the Limonium gmelinii roots [1]. As previously shown, iron oxide nanoparticles are able to reactivate immune cells [2], so, we synthesized them in our extract.

Growth of sarcoma 45 was accompanied by a sharp increase in the content of lactic acid (LA) in almost all analyzed organs. Significant decrease from 17.04 to 0.3 mmol/g after Limonidin application was found in the kidneys, 1.5 times decrease noted in the liver and 2–3 times decrease in the lungs, heart and skeletal muscle. Limonidin contributed to the restoration of normal LA content in most of the internal organs, especially in the parenchymal organs – in the kidneys, liver and spleen. Tumor growth was characterized by a decrease in the content of pyruvic acid by 50-60% in the liver and heart of tumor carriers, and in the kidneys and lungs its content decreased by 30-50% with insignificant fluctuations in the spleen. It was noted that Limonidin contributes to an increase in the content of pyruvic acid in the liver, spleen and muscles by 30-40%, in the tumor – by 70%, in the lungs, heart and kidneys – by 2–4.2 times. Based on our results, we may say that the drug corrects the disturbed metabolism of tumor cells.

• References

• 1 State Pharmacopeia of the Republic of Kazakhstan vol. 2. Almaty: Zhibek zholy” publ; 2009: 706-707.
• 2 Zanganeh S, Hutter G, Spitler R. , et al. Iron oxide nanoparticles inhibit tumour growth by inducing pro-inflammatory macrophage polarization in tumour tissues. Nat Nanotechnol 2016; 11 (11) : 986-994.