Planta Med 2019; 85(18): 1460-1461
DOI: 10.1055/s-0039-3399826
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Cutting-edge analytical technologies for the comprehensive metabolic profiling of Alkanna tinctoria roots cultured in greenhouse conditions

N Rodić
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and NatPro-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
,
A Vlachou
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and NatPro-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
,
C Papakonstantinou
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and NatPro-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
,
Y Zhao
2   Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain,, Croix du Sud 2, box L7.05.06, 1348, Louvain-la-Neuve, Belgium
,
A Cartabia
2   Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain,, Croix du Sud 2, box L7.05.06, 1348, Louvain-la-Neuve, Belgium
,
I Lalaymia
2   Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain,, Croix du Sud 2, box L7.05.06, 1348, Louvain-la-Neuve, Belgium
,
K Grigoriadou
3   Laboratory for Conservation and Evaluation of Native and Floricultural Species-Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter,, P.O. Box 60458, GR-57001 Thermi, Thessaloniki, Greece
,
N Krigas
3   Laboratory for Conservation and Evaluation of Native and Floricultural Species-Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter,, P.O. Box 60458, GR-57001 Thermi, Thessaloniki, Greece
,
E Maloupa
3   Laboratory for Conservation and Evaluation of Native and Floricultural Species-Balkan Botanic Garden of Kroussia, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter,, P.O. Box 60458, GR-57001 Thermi, Thessaloniki, Greece
,
S Declerck
2   Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain,, Croix du Sud 2, box L7.05.06, 1348, Louvain-la-Neuve, Belgium
,
HG Gika
4   School of Medicine, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and BIOMIC-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
,
VP Papageorgiou
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and NatPro-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
,
AN Assimopoulou
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 541 24, Thessaloniki, Greece and NatPro-AUTH, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center,, Buildings A & B, 10th km Thessaloniki-Thermi Rd, P.O. Box 8318, GR 570 01, Thessaloniki, Greece
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

Also available at
 

The use of plants containing naphthoquinone derivatives Alkannins & Shikonins (A/S) by humans dates back to ancient times. In recent decades, the use of A/S has seen a resurgence and A/S have risen to a pivotal role as pharmaceutical and cosmeceutical agents, since they possess strong wound healing, antimicrobial, anti-inflammatory, tissue regenerative and antitumor properties. It is thus crucial to enhance the biosynthesis of bioactive A/S in Alkanna tinctoria plants, that naturally produce high amounts of these metabolites [1].

In the frame of “MICROMETABOLITE” EU H2020 project, we have optimized a workflow for the metabolic profiling of A. tinctoria roots, cultured in the greenhouse from plants obtained by in vivo shoot cuttings. A fast and reliable extraction procedure was achieved for comprehensive profiling and identification of A/S and other metabolites biosynthesized in the roots. The aim of this work was to determine the growth stage with peak A/S production, while simultaneously obtaining additional information on the root metabolome. A combination of UHPLC-HRMS and NMR was used for metabolite identification, HPLC was utilized for reliable quantitation of A/S and the extracts were subjected to chiral HPLC analysis [2] for determination of the enantiomeric A/S ratio.

Different A/S derivatives and other metabolites were identified in plant roots using UHPLC-HRMS and NMR. Six A/S derivatives and total A/S were quantified using HPLC-DAD. From six vegetation stages of A. tinctoria grown under greenhouse conditions, fruiting period was found to peak A/S production (1% wt/wt of root), while the enantiomeric alkannin/shikonin ratio remained constant (93.7%).

 

  • References

  • 1 Papageorgiou VP, Assimopoulou AN, Couladouros EA, Hepworth D, Nicolaou KC. The chemistry and biology of alkannin, shikonin, and related naphthazarin natural products. Angew Chemie - Int Ed 1999; 38: 270-300
    CrossrefPubMedGoogle Scholar
  • 2 Tappeiner J, Vasiliou A, Ganzera M, Fessas D, Stuppner H, Papageorgiou VP, Assimopoulou AN. Quantitative determination of alkannins and shikonins in endemic Mediterranean Alkanna species. Biomed Chromatogr 2014; 28 (07) 923-933
    CrossrefPubMedGoogle Scholar