Planta Med 2019; 85(18): 1466
DOI: 10.1055/s-0039-3399841
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Plant extracellular vesicle lipids and secondary metabolites

E Woith
1   Freie Universität Berlin, Institute of Pharmacy - Pharmaceutical Biology, Dahlem Centre of Plant Sciences,, Königin-Luise-Str. 2+4, 14195 Berlin, Germany
,
A Jendretzki
1   Freie Universität Berlin, Institute of Pharmacy - Pharmaceutical Biology, Dahlem Centre of Plant Sciences,, Königin-Luise-Str. 2+4, 14195 Berlin, Germany
,
A von zur Gathen
1   Freie Universität Berlin, Institute of Pharmacy - Pharmaceutical Biology, Dahlem Centre of Plant Sciences,, Königin-Luise-Str. 2+4, 14195 Berlin, Germany
,
A Janik-Polanowicz
2   Wroclaw Medical University, Department of Pharmaceutical Biology,, 50375, Wrocław, Poland
,
MF Melzig
1   Freie Universität Berlin, Institute of Pharmacy - Pharmaceutical Biology, Dahlem Centre of Plant Sciences,, Königin-Luise-Str. 2+4, 14195 Berlin, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Extracellular vesicles (EVs) are lipid bilayer delimited vehicles, carrying small noncoding RNAs, besides other bioactive compounds [1]. While plenty of information is available on mammalian EVs, our knowledge on plant EVs remains little. Their effects on human body are currently being investigated, while it is still unexplored how EV lipids vary between distinct species or whether secondary metabolites such as polyphenols or alkaloids are packaged into plant derived EVs. Due to manifold differences in plant families, regarding secondary metabolites and structural characteristics, the lipid composition of EV membranes probably also varies. In order to get insight into the lipid composition of EVs, an extraction method was developed and lipophilic compounds profiled by TLC. In general, EV fractions, isolated by differential centrifugation, were shown to enrich the added membrane dye 3,3′-Dihexyloxacarbocyanine iodide (DiOC6) compared to the supernatant. Further, preliminary data suggest phosphatidylserine to be present in EV preparations from Hypericum perforatum L. and Viscum album L. It is still puzzling how plant EVs act in gastrointestinal environment. Since secondary metabolites such as polyphenols have the potential to inhibit gastrointestinal enzymes [2] and thereby prevent EV degradation, we investigated if secondary metabolites are packaged into EVs. Although TLC analysis revealed the abundance of several not yet identified structures, nicotine was absent in EV preparations from Nicotiana tabacum L. callus culture.

 

  • References

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