Planta Med 2019; 85(18): 1479
DOI: 10.1055/s-0039-3399872
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Combined HPLC-PDA-MS and NMR study of Matricaria pubescens extracts from Algeria

ME Ignatiadou
1   Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki,, University Campus, 54124, Thessaloniki, Greece
,
S Zoumpou
1   Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki,, University Campus, 54124, Thessaloniki, Greece
,
Z Kabouche
2   Laboratoire d’Obtention de Substances Therapeutiques (L.O.S.T), Faculté des Sciences, Université Mentouri-Constantine,, Campus Chaabat Ersas, 25000, Constantine, Algerie
,
A Karioti
1   Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki,, University Campus, 54124, Thessaloniki, Greece
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
20. Dezember 2019 (online)

Auch verfügbar auf
 

Matricaria pubescens (Desf.) Schultz (Asteraceae), known as hairy chamomile, is endemic in North Africa, especially in the Algerian Sahara and is used for several ailments such as rheumatic and muscular pains, coughs, allergies, ocular affections, dysmenorrhoea, scorpion stings, dehydration and toothaches [1]. Previous phytochemical studies on this species had revealed the presence of flavonoids and coumarins [2]. As a part of a project aiming at studying Matricaria sp. the analysis of M. pubescens was undertaken, but this time through an integrated chemical profiling combining HPLC-PDA-MS, NMR and phytochemical analyses, where appropriate. A medium polarity extract was prepared according to the Bohlmann protocol (cyclohexane:diethylether:methanol 1:1:1), slightly modified [3]. This extract was further subjected to fractionation and all separation steps thereof were monitored by both HPLC-PDA-MS and NMR. Analyzes did not show the presence of sesquiterpene lactones, but instead evidenced several flavonoids and phenolic compounds. More than 20 compounds have been unambiguously identified up to now, among them luteolin-4ʹ-O-glucopyranoside and quercetagenin-3-O-glucopyranoside, reported for the first time in Matricaria sp. Furthermore, two polyamines previously reported in other Asteraceae sp. [4],[5] have been isolated and characterized. Fingerprint analysis and comparison of this species to the officially recognized in Europe M. recutita L. shows many similarities in the chemical content and justifies the ethnopharmacological uses in Algerian traditional medicine.

 

  • References

  • 1 Cherif HS, Ferrah R, Bennacer A, Tail G, Saidi F. Traditional use of Matricaria pubescens (Desf.) Schultz in two regions of southern Algeria and contribution to study the antioxidant activity. Indian J Trad Knowl 2017; 16: 562-567
    PubMedGoogle Scholar
  • 2 Gherboudj O, Benkiki N, Seguin E, Tillequin F, Kabouche Z. Components of Matricaria pubescens from Algerian septentrional Sahara. Chem Nat Comp 2012; 48: 470-471
    CrossrefPubMedGoogle Scholar
  • 3 Saroglou V, Karioti A, Heilmann J, Kypriotakis Z, Skaltsa H. Sesquiterpene Lactones from Anthemis melanolepis . Helv Chim Acta , 2007; 90: 171-175
    CrossrefPubMedGoogle Scholar
  • 4 Park SB, Song K, Kim YS. Tetra-cis/trans-Coumaroyl Polyamines as NK1 receptor antagonists from Matricaria chamomilla . Planta Med Int Open 2017; 4: e43-e51
    PubMedGoogle Scholar
  • 5 Yamamoto A, Nakamura K, Furukawa K, Konishi Y, Ogino T, Higashiura K. et al. A new nonpeptide tachykinin NK1 receptor antagonist isolated from the plants of compositae. Chem Pharm Bull 2002; 50: 47-52
    CrossrefPubMedGoogle Scholar