Extraction of active compounds of Sedum roseum by natural deep eutectic solvent

Sedum roseum (L.) Scop. (syn. Rhodiola rosea L.) is well established phytoadaptogen used in traditional and officinal medicine all over the world [1],[2]. Ethanol is most common solvent for the extraction of active compounds from plants. Natural deep eutectic solvents (NDES) are emerging as green and sustainable solvents for efficient extraction of bioactive compounds and as alternative to organic solvents. NDES ultrasonic assisted extraction was developed to extract roots and rhizomes of S.roseum. The concentrations of salidroside, rosin, rosavin, tyrosol, and cinnamic alcohol were analysed by HPLC-DAD. NDES have been prepared using mixtures of glucose, fructose, and lactic acid. In result of optimization of extraction conditions the total concentration of 5 analysed compounds in NDES extract was equivalent to those in 40% EtOH extract. The solubility of glycosides in NDES was higher than those of tyrosol, and cinnamic alcohol. This fact could be explained by polarity of solvent used. However, by variation of composition of NDES we were able to receive extracts with increased concentration of tyrosol, and cinnamic alcohol, while the concentration of glycosides was a little decreased.

To the best of our knowledge this is a first report in which NDES was used for effective extraction of S.roseum.

• References

• 1 Panossian A, Wikman G, Sarris J. Rosenroot (Rhodiola rosea): traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 2010; 17: 481-493.
• 2 Shikov AN, Pozharitskaya ON, Makarov VG, Wagner H, Verpoorte R, Heinrich M. Medicinal plants of the Russian Pharmacopoeia; their history and applications. J Ethnopharmacol 2014; 154: 481-536.