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DOI: 10.1055/s-0039-3399939
Unprecedented isoflavanoid-neoflavonoid analogues isolated from Brazilian red propolis
Publication History
Publication Date:
20 December 2019 (online)
Propolis is a natural resinous product collected by honeybees from the buds and exudates of various plant sources, which has been used as a traditional remedy in folk medicine for centuries. Among Brazilian propolis varieties, Brazilian red propolis (BRP) is isoflavonoid-rich. Bees producing red propolis feed upon Dalbergia ecastophyllum [1]–[4] In this investigation, BRP was extracted with 80% EtOH shaken at 70°C for 30 min. After extraction, the mixture was centrifuged. The supernatant was evaporated to dryness to give a EtOH extract of Brazilian red propolis (EERP) [1],[2]
The EERP extract was fractionated by normal phase silica gel column eluted with a linear gradient of CHCl3/EtOAc. Fraction 5 was separated by C18 RPCC and eluted with a linear gradient of MeOH in H2O. Fractions collected were evaporated to dryness, resulting in thirty fractions. After successive fractionations, eight structurally novel isoflavanoid-neoflavonoid analogues were obtained and identified by extensive analysis of spectroscopic data. The cytotoxic and anti-inflammatory activities of isoflavanoid-neoflavonoid analogues are under evaluation.
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References
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