Planta Med 2019; 85(18): 1526
DOI: 10.1055/s-0039-3400028
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Effects of different essential oils on HaCaT keratinocytes against hydrogen peroxide induced oxidative stress

E Neza
1   Department of Pharmacy, Aldent University,, Rruga e Dibres, Nr.235, Tirane, Albania
,
M Centini
2   Department of Biotechnologies, Chemistry and Pharmacy, University of Siena,, Via Aldo Moro 2, 53100 Siena, Italy
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Many essential oils have found applications in pharmaceutical and cosmetic products. They are frequently used as antibacterial, antifungal and antiviral and antioxidant compounds. Essential oils may exhibit cytotoxic effects on skin cells. They can sometime cause skin irritations and allergic reactions. Citronellol, citral, cinnamal, geraniol, linalool, d-limonene, eugenol, hydroxyl-citronellol are some essential oils compounds with well-known allergenic properties.

The effect of different concentrations (0.001, 0.01, 0.1 and 1% (v/v)) of common juniper (Juniperus communis), oregano (Origanum vulgare), laurel (Laurus nobilis), lavender (Lavandula officinalis), sage (Salvia officinalis) and myrtle (Myrtus communis) essential oils on human skin cells HaCaT in vitro was explored. Essential oils effect on hydrogen peroxide (H2O2)-treated keratinocyte HaCaT cells were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Essential oils were extracted with hydrodistillation.

This study has demonstrated that all essential oils were cytotoxic to human keratinocytes in vitro at a concentration of 1% (V/V). At a concentration of 0.1%, all essential oils, with the exception of Laurus nobilis L., were still irritating. At lower concentrations all essential oils showed no toxicity and no protective effect on HaCaT human keratinocytes from oxidative stress induced by exposure to hydrogen peroxide.

 

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