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DOI: 10.1055/s-0039-3400043
HPLC-based activity profiling of Haplophyllum tuberculatum In vitro activity against Madurella mycetomatis
Publication History
Publication Date:
20 December 2019 (online)
Eumycetoma is a chronic debilitating inflammatory fungal infection caused mainly by Madurella mycetomatis. Eumycetoma is endemic in most tropical and subtropical countries and was recently enlisted by the WHO among the neglected tropical diseases (NTDs) [1]. Current treatment includes long-term treatment with itraconazole and surgical intervention in most of the cases. Given limited efficacy, frequent side effects, and high cost of treatment, there is an urgent need for new antimycetomal drugs.
A subset of extracts from a repository of Sudanese medicinal plants traditionally used as anti-infectives was screened in a 96-well microtitre assay for in vitro activity against M. mycetomatis employing resazurin viability assay [2].
The chloroform extract from roots of Haplophyllum tuberculatum (Forsskal) A. Juss. (Rutaceae) was found to be the most active. Activity in the extract was localized by HPLC-based activity profiling, and two lignans, nectandrin B (1) and tetrahydrofuroguaiacin B (2), were isolated from the active time window.
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References
- 1 van de Sande W, Fahal A, Ahmed SA, Serrano JA, Bonifaz A, Zijlstra E. et al. Closing the mycetoma knowledge gap. Med Mycol 2018; Apr 1;56 (suppl_1): 153–64 DOI: 10.1093/mmy/myx061..
- 2 Khalid SA. Development of microtiter plate-based method for the determination of the MIC of antimycetomal agents against Madurella mycetomatis. II ResNet NPND workshop on natural products against neglected diseases, Nov. 25 – 28th, 2014, Rio de Janeiro, Brazil.