Planta Med 2019; 85(18): 1533-1534
DOI: 10.1055/s-0039-3400047
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Immunomodulatory effect of Acacia nilotica pods on Leishmania parasitized THP-1 macrophage cells

NK Mustafa
1   Institue of Endemic Diseases, University of Khartoum,, Khartoum, Sudan
,
MM Mukhtar
1   Institue of Endemic Diseases, University of Khartoum,, Khartoum, Sudan
,
Al Dawi AF
1   Institue of Endemic Diseases, University of Khartoum,, Khartoum, Sudan
,
S Khalid
2   University of Khartoum,, Faculty of Pharmacy, Sudan
3   Faculty of Pharmacy, University of Science & Technology Omdurman, Sudan
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Leishmaniasis is a neglected disease with an estimated 12 million humans infected and there is an urgent need for alternative novel drugs. Acacia nilotica (L.) Willd. Ex Del (Fabaceae) is frequently used in treating parasitic disease in Sudan. Analysis of the ethyl acetate fraction of Acacia nilotica pods revealed its high contents of both hydrolysable and condensed tannins [1].

The in vitro growth inhibition and THP-1 macrophage activation of the ethyl acetate (EtOAc) and chloroform (CHCl3) fractions of A. nilotica pods on L. donovani and L. major were assessed by measuring the production of TNF-α, IL-6, IL-β cytokines and nitric oxide. The EtOAc fraction inhibited significantly (P < 0.05) the growth rate of L. donovani and L. major promastigote with IC50 of 40μg/ml and 10μg/ml, respectively.

EtOAc fraction also inhibited the amastigotes in L. donovani and L. major, in a dose dependent response, with an IC50 of 10ug/ml and 100 ug/ml compared with pentostam and amphotericin B, respectively. EtOAc fraction caused significantly higher levels of IL-6 coupled with lowering TNF-α and IL-β levels in the infected macrophages of both Leishmania species [Fig. 1]. The nitric oxide exhibited highest level (1000ug/ml) in comparison with the blank. However, the CHCl3 fraction was almost devoid of any significant effect. The standard drugs’ IC50 were ranging between ˃24µg/ml and ˃10µg/ml. The present results demonstrate the viability of THP-1 macrophage as model to study the immune modulatory effects of tannins by macrophage activation via extra- and intra-cellular release of cytokines [2].

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Fig. 1 TNF-α, IL-β and IL-6 concentrations in supernatants of THP-1 Cell lines infected with Leishmania donovani (VL) and Leishmania major (CL) and treated with different concentrations of Ethyl acetate fractions of Acacia nilotica.
 

  • References

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