Planta Med 2019; 85(18): 1563
DOI: 10.1055/s-0039-3400134
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Antimicrobial and cytotoxic properties of the Copaifera reticulata oleoresin and its major diterpene acids

AL Pfeifer Barbosa
1   Institute of Pharmacy, Department of Pharmaceutical Biology, Kiel University,, Gutenbergstraße 76, Kiel, Germany
2   Institute of Pharmacy, Department of Pharmaceutical and Medicinal Chemistry, Kiel University,, Gutenbergstraße 76, Kiel, Germany
,
A Wenzel-Storjohann
3   GEOMAR Centre for Marine Biotechnology, Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel,, Am Kiel-Kanal 44, 24106 Kiel, Germany
,
JD Barbosa
4>   Faculdade de Medicina Veterinaria, Central de Diagnostico Veterinario, Universidade Federal do Para, Campus Castanhal,, Rua Maximino Porpino da Silva, 1000, 68710-080 Castanhal, PA, Brazil
,
C Zidorn
1   Institute of Pharmacy, Department of Pharmaceutical Biology, Kiel University,, Gutenbergstraße 76, Kiel, Germany
,
C Peifer
2   Institute of Pharmacy, Department of Pharmaceutical and Medicinal Chemistry, Kiel University,, Gutenbergstraße 76, Kiel, Germany
,
D Tasdemir
3   GEOMAR Centre for Marine Biotechnology, Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel,, Am Kiel-Kanal 44, 24106 Kiel, Germany
5   Kiel University,, 24118 Kiel, Germany
,
SS Ҫiçek
1   Institute of Pharmacy, Department of Pharmaceutical Biology, Kiel University,, Gutenbergstraße 76, Kiel, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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The oleoresin of Brazilian Copaifera reticulata (Fabaceae) is traditionally used for the treatment of skin and urinary tract infections, respiratory diseases, rheumatism, ulcer and tumors. Thus, playing an important role in the primary health care of the indigenous population [1],[2].

As previous pharmacological tests used the crude oleoresin and only a few studies dealt with pure chemically defined compounds, the aim of this study was to systematically evaluate the antimicrobial and cytotoxic properties of the oleoresin and to assign traditional uses to specific secondary metabolites.

Whereas no cytotoxic activity was detected, the oleoresin showed activity against gram-positive bacteria Enterococcus faecium (IC50 value of 4.2 μg/mL) and methicillin-resistant Staphylococcus aureus (MRSA, IC50 value of 5.3 μg/mL). Fractionation of the oleoresin yielded two dicarboxylic diterpene acids and the four major diterpene acids, comprising three different diterpene scaffolds. Interestingly, the activity was not restricted to a certain diterpene-type but rather correlated with the compounds’ lipophilicity, with the most active compound, (13E)-ent-labda-7,13-dien-15-oic acid, displaying IC50 values of 1.6 (E. faecium) and 2.5 μg/mL (MRSA), respectively. The major diterpenoid, ent-polyalthic acid, was significantly active against dermatophytes with IC50 values of 6.8 μg/mL (Trichophyton rubrum) and 4.3 μg/mL (T. mentagrophytes).

The present study proved the antimicrobial effects of the C. reticulata oleoresin and its diterpenoid constituents, supporting its wide use in folk medicine for the treatment of skin and urinary tract infections and provides potential lead structures for the treatment of two clinically relevant bacterial strains.

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Fig. 1
 

  • References

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