Reduction of an in vitro Intraradicular Multispecies Biofilm Using Two Rotary Instrumentation Sequences

Angelo Zavattini; Jonathan Cowie; Sadia Niazi; Massimo Giovarruscio; Salvatore Sauro; Federico Foschi

doi:10.1055/s-0040-1701541

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(01): 001-007
DOI: 10.1055/s-0040-1701541

Original Article

Reduction of an in vitro Intraradicular Multispecies Biofilm Using Two Rotary Instrumentation Sequences

Angelo Zavattini

¹Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Postgraduate Centre, Guy's Hospital, London Bridge, King's College London, London, United Kingdom

,

Jonathan Cowie

²Circus Dental Practice, Bath, United Kingdom

,

Sadia Niazi

¹Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Postgraduate Centre, Guy's Hospital, London Bridge, King's College London, London, United Kingdom

,

Massimo Giovarruscio

¹Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Postgraduate Centre, Guy's Hospital, London Bridge, King's College London, London, United Kingdom

³Department of Therapeutic Dentistry, I.M. Sechenov First Moscow State Medical University, Moscow, Russia

,

Salvatore Sauro

³Department of Therapeutic Dentistry, I.M. Sechenov First Moscow State Medical University, Moscow, Russia

⁴Departamento de Odontologia, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Valencia, Spain

,

Federico Foschi

¹Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Postgraduate Centre, Guy's Hospital, London Bridge, King's College London, London, United Kingdom

³Department of Therapeutic Dentistry, I.M. Sechenov First Moscow State Medical University, Moscow, Russia

› Author Affiliations

Abstract

Objective The purpose of this research was to investigate the effect per se of two shaping and cleaning techniques on the reduction of an in vitro multispecies biofilm.

Materials and Methods A total of 39 freshly extracted monoradicular teeth for periodontal reason were decoronated. Roots were sectioned longitudinally. After autoclaving, a specific stressed biofilm was grown on the root halves that were subsequently reassembled in a silicone index. Two treatments (n = 9 each)—RaCe (Schottlander; Letchworth Garden City, United Kingdom) and ProTaper Gold ( PTG; Dentsply Maillefer, Baillagues, Switzerland)—were tested; three noninstrumented samples served as a control group and three were rinsed with saline. Posttreatment samples were taken at three different levels of the root. Colony-forming units were counted after incubations. Additionally, three treatments (n = 5 each)—RaCe, PTG, and saline only—were evaluated under a confocal laser scanning microscope (CLSM).

Statistical Analysis Statistical analysis was conducted using Tukey’s test and analysis of variance to evaluate the post-instrumentation bioburden.

Results Both instrumentations were able to reduce the biofilm; however, differences were not present between them (p > 0.05). CLSM showed biofilm killing and disruption through mechanical shaping alone.

Conclusions Intraradicular biofilm is reduced with mechanical shaping. There was no difference between RaCe and PTG systems in biofilm reduction despite differences in design, file sequence, and rotational speed.

Keywords

biofilm - chemomechanical preparation - confocal microscope - endodontic infection - ProTaper Gold - RaCe - root canal

Full Text

References

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