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DOI: 10.1055/s-0040-1701846
Increased Expression of Matrix Metalloproteinases 2 and 9 as Poor Prognosis Factor for Hodgkin’s Lymphoma Patients
Publikationsverlauf
Publikationsdatum:
18. März 2020 (online)
Introduction Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases that act on both renewal and remodeling of the extracellular matrix. Increased expression of these proteins is associated with neoplastic, invasive and metastatic processes. Studies show that in Hodgkin’s Lymphoma (HL), strong expression of metalloproteinase 2 (MMP-2) correlates with a favorable prognosis, while expression of metalloproteinase 9 (MMP-9) showed an adverse outcome. Therefore, the aim of our study was to evaluate the expression of MMP-2 and MMP-9 as a prognostic factor in patients diagnosed with HL.
Methods In the present study, 45 paraffin biopsies from patients up to 19 years old diagnosed with HL were used in two referral hospitals in the state of Pernambuco, Brazil. The immunohistochemistry reaction used primary monoclonal antibodies directed to matrix metalloproteinases (MMP-2 and -9) to detect such proteins in the tumor microenvironment.
Results MMP-2 intensity pattern was stronger (> 10% of total field) in patients with stage III/IV and B symptoms. MMP-2 x Risk group showed significant value (p=0,0388). That is, the stronger the MMP-2 marking, the greater the unfavorable risk. However for MMP-9 there was no difference in the stronger intensity pattern in relation to stages I/II and III/IV, only in the presence of symptoms B. The criterion MMP-9 x Symptoms B showed significant value (p = 0.0411). Therefore, patients with B symptoms have higher MMP-9 expression.
Conclusion Thus, we believe that both MMP-2 and MMP-9 can be considered poor prognostic molecules for Hodgkin’s lymphoma and the that RNA expression evaluation could confirm the results.
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