Endoscopy 2020; 52(S 01): S30
DOI: 10.1055/s-0040-1704094
ESGE Days 2020 oral presentations
Friday, April 24, 2020 17:00 – 18:30 Advances in endoluminal and biliopancreatic endoscopy The Liffey A
© Georg Thieme Verlag KG Stuttgart · New York

METALLIC STENT MESH COATED WITH AG NANOPARTICLE SUPPRESSES STENT-INDUCED TISSUE HYPERPLASIA AND BILIARY SLUDGE IN RABBIT EXTRAHEPATIC BILE DUCT

JH Park
1   Asan Medical Center, University of Ulsan College of Medicine, Radiology and Research Institute of Radiology, Seoul, Korea, Republic of
,
SH Kim
2   Jeonbuk National University Hospital, Internal Medicine, Jeonju, Korea, Republic of
,
KY Kim
3   Jeonbuk National University Hospital, Radiology, Jeonju, Korea, Republic of
,
ST Lee
2   Jeonbuk National University Hospital, Internal Medicine, Jeonju, Korea, Republic of
,
SO Lee
2   Jeonbuk National University Hospital, Internal Medicine, Jeonju, Korea, Republic of
› Author Affiliations
Further Information

Publication History

Publication Date:
23 April 2020 (online)

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Aims Current therapeutic strategies are insufficient for suppressing stent restenosis after biliary stent placement. The effects of silver nanoparticles (AgNPs) coated metallic stent mesh for suppression of stent-induced tissue hyperplasia and biliary sludge formation in the rabbit extrahepatic bile duct were investigated.

Methods Meshes of self- expandable metal stent (SEMS) were coated with three different concentrations of AgNP to enable antibacterial and anti-inflammatory activities. Twenty-four rabbits were randomly divided into four groups of six each. Group A received bare uncoated SEMS. Groups B, C, and D received AgNP-coated SEMS of 3, 6, and 12 mg/mL of Ag concentration. The effectiveness of AgNP-coated SEMS was accessed by comparing the results of cholangiography, gross, and histological examination.

Results AgNP-coated SEMSs were successfully fabricated using two-step synthesis. SEMS placement was technically successful in 22 of 24 rabbits. Two rabbits were excluded because of procedure-related death. Sludge formation in AgNP-coated SEMS groups was prominently decreased compared with the control group on gross findings. Cholangiographic and histologic examinations revealed significantly less stent-induced tissue hyperplasia in AgNP-coated SEMS groups than in the control group (p < 0.05 for all). There were no significant differences of cholangiographic stenosis or percentage of granulation tissue area between AgNP-coated SEMS groups (p > 0.05 for all). However, the thickness of submucosal fibrosis in group D was higher than that of groups B and C (p < 0.05 for all).

Conclusions SEMS with AgNP-coated stent mesh suppressed stent-induced tissue hyperplasia and biliary sludge formation in the rabbit common bile duct. AgNP concentrations of 3 to 6 mg/mL are preferable for Ag nano-functionalized SEMS to prevent these phenomena.