Nuklearmedizin 2020; 59(02): 116
DOI: 10.1055/s-0040-1708198
Wissenschaftliche Vorträge
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© Georg Thieme Verlag KG Stuttgart · New York

The interaction of lung cancer and adipose tissue in the development of cancer cachexia

A Frille
1   University of Leipzig, Department of Respiratory Medicine, IFB AdiposityDiseases, Leipzig, Germany
,
N Linder
2   University of Leipzig, Department of Diagnostic and Interventional Radiology, IFB AdiposityDiseases, Leipzig, Germany
,
J Pappisch
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
T Kerkhoff
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
J Meyer
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
H Kuhn
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
C Hänel
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
H Busse
2   University of Leipzig, Department of Diagnostic and Interventional Radiology, IFB AdiposityDiseases, Leipzig, Germany
,
K. G Steinhoff
4   University of Leipzig, Department of Nuclear Medicine, Leipzig, Germany
,
M Rullmann
5   University of Leipzig, Department of Nuclear Medicine, IFB AdiposityDiseases, Leipzig, Germany
,
T Ebert
6   University of Leipzig, Medical Department III – Endocrinology, Nephrology, Rheumatology, IFB AdiposityDiseases, Leipzig, Germany, Karolinska Institutet, Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Renal Medicine, Stockholm, Sweden
,
H.J Seyfarth
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
O Sabri
5   University of Leipzig, Department of Nuclear Medicine, IFB AdiposityDiseases, Leipzig, Germany
,
H Wirtz
3   University of Leipzig, Department of Respiratory Medicine, Leipzig, Germany
,
S Hesse
5   University of Leipzig, Department of Nuclear Medicine, IFB AdiposityDiseases, Leipzig, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
08 April 2020 (online)

 

Ziel/Aim Lung cancer patients (LCP) often experience cancer cachexia (CC). Brown (BAT) and white adipose tissue (WAT) may play a role in the development of CC and resistance to chemotherapy. In a translational approach, we aimed to find out whether and how adipose tissue interacts with LC in cachexia.

Methodik/Methods Retrospectively, 200 LCP and 30 healthy controls (HC) were analyzed for BAT activation via fluorine-18 deoxyglucose positron emission tomography/computed tomography. Mean standardized uptake values (SUVmean) of predefined regions of interest (ROI) in the retroclavicular fat were measured, normalized to liver uptake and reported as SUV ratio (SUVR). ROIs from transversal CT image were used to quantify visceral adipose (VAT) and muscle mass. Prospectively, 50 LCP were likewise analyzed for BAT activation and additionally underwent bioelectrical impedance analysis to assess body composition and analysis of circulating adipokines. In an in-vitro co-culture system, the interactions between LC cell lines (H322, A549, H1650, PC9) and BAT or WAT were analyzed for resistance to the chemotherapeutics cisplatin, gefitinib, or osimertinib via MTT assay and for specific adipokine signaling.

Ergebnisse/Results LCP showed higher SUVR in BAT than the HC. Higher SUVR in BAT was associated with lower BMI (r = −0.38) and reduced VAT (r = −0.44). LCP with higher SUVR in BAT were associated with significant weight loss (odds ratio 2.3, P <  0.05). These results were confirmed in the prospective cohort. Circulating adiponectin levels positively correlated with SUVR in BAT (r = 0.71) and inversely with both BMI (r = −0.61), VAT (r = −0.58) and lean body mass (r = −0.53). Co-cultured adipocytes reduced chemosensitivity in lung cancer cells.

Schlussfolgerungen/Conclusions LCP express higher BAT activity than HC, which is correlated with CC. Adiponectin is associated with BAT activation and leaner body composition. At the cellular level, co-cultured adipocytes can lead to resistance to chemotherapy in LC cells.