Abstract
Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa
β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound.
Materials and Methods An experimental study with post-test only control group design was conducted. Twenty
male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with
MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE
50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical
analysis for the expressions of TNF-α and NF-κB.
Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79),
while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile
the comparison between treatment groups did not highlight any significant difference
(p > 0.05). The highest expression of NF-κB was observed in the control group (13.20
± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14).
NF-κB was significantly lower in the treatment group using MASE 50% when compared
with other treatment groups (p < 0.05).
Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at
all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.
Keywords
Musa acuminata
- anti-inflammation - TNF-α - NF-κB
