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CC BY 4.0 · Journal of Child Science 2021; 11(01): e338-e349
DOI: 10.1055/s-0040-1718543
Original Article

Epidemiology of Group A Rotavirus Diarrhea among Children Hospitalized for Acute Gastroenteritis in Ondo State, Nigeria

Michael Oluyemi Babalola
1   Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria
2   Department of Microbiology, Division of Infectious Diseases Epidemiology, Virology and Special Pathogens Research, Adekunle Ajasin University, Ondo State, Nigeria
,
David Olufemi Olaleye
1   Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria
,
Georgina Njideka Odaibo
1   Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria
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Abstract

Globally, infective group A rotavirus (RVA) enteric infection in children culminates in acute diarrheal disease, severe dehydration, and mortality in children under the age of 5 years, particularly in sub-Saharan Africa. This research sought to determine the prevalence of RVA diarrhea among children aged below 5 years in Ondo state, as one of the necessary frameworks before instituting a vaccine campaign, and to expand knowledge on the epidemiology of RVA diarrhea in Nigeria.

In a cross-sectional descriptive study between October 2012 and September 2014, convenience sampling was adopted to obtain demographic information, clinical details, and stool samples from accented under five children who sought treatment for acute gastroenteritis at designated hospitals in Akure and Owo, Nigeria. A total of 390 stool samples were collected from children with acute diarrhea and tested for VP6 RVA antigen using enzyme immunoassay. Data generated were analyzed using descriptive statistics and chi-square at α 0.05.

From the 390 children hospitalized for diarrhea, 240 samples (240/390; 61.5%) were from males, while 150 samples (150/390; 38.5%) were from females, representing a male:female ratio of 1.6:1. RVAs were found in 24.2% (58/240) males and 28% (42/150) females, giving a male-female ratio of 1:1.2 and total prevalence of 25.6% (100/390). RVA infection was inversely proportional to the age as a rate of 11.8% was observed in children aged above 36 months and 31.8% (35/110) in children 7 to 12 months, while the highest rate (45.7%) was among children ≤ 6 months old. No significant difference was found (chi-square = 0.712) in the induction of diarrhea in children from Akure and Owo, neither was there any significant difference in the rates of infection between the boys and girls in Akure (chi-square = 0.576) nor in Owo (chi-square = 0.333). Seasonal association (chi-square = 5.802) in RVA infection was observed in the rainy season of year 2013/2014 period.

RVA diarrhea occurred year-round at a prevalence of 25.6%, predominantly in females. A seasonal fluctuation was observed in the rainy and dry seasons of the 2-year period. RVA diarrhea occurred predominantly in children aged below 18 months of age, and may thus help in determining the optimal period/schedule of any immunization program.

Keywords

diarrhea - RVA - epidemiology - children - Ondo state - Nigeria

Note

This publication derived from the PhD Research Thesis of M.O.B. on molecular epidemiology of group A rotavirus among children with gastroenteritis in Ondo state, Nigeria.




Publication History

Received: 30 May 2020

Accepted: 17 August 2020

Article published online:
25 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Greenberg HB, Estes MK. Rotaviruses: from pathogenesis to vaccination. Gastroenterology 2009; 136 (06) 1939-1951
    CrossrefPubMedGoogle Scholar
  • 2 Estes MK, Greenberg HB. Rotaviruses. In: Knipe DM, Howley PM. et al, eds. Fields Virology, 6th ed. Philadelphia, PA: Wolter Kluwer Health/Lippincott Williams & Wilkins; 2013: 1347-1401
    Google Scholar
  • 3 Mihalov-Kovács E, Gellért Á, Marton S. et al. Candidate new rotavirus species in sheltered dogs, Hungary. Emerg Infect Dis 2015; 21 (04) 660-663
    CrossrefPubMedGoogle Scholar
  • 4 Bányai K, Kemenesi G, Budinski I. et al. Candidate new rotavirus species in Schreiber's bats, Serbia. Infect Genet Evol 2017; 48: 19-26
    CrossrefPubMedGoogle Scholar
  • 5 Saif LJ, Jiang B. Nongroup A rotaviruses of humans and animals. Curr Top Microbiol Immunol 1994; 185: 339-371
    PubMedGoogle Scholar
  • 6 Doan LT, Okitsu S, Nishio O, Pham DT, Nguyen DH, Ushijima H. Epidemiological features of rotavirus infection among hospitalized children with gastroenteristis in Ho Chi Minh City, Vietnam. J Med Virol 2003; 69 (04) 588-594
    CrossrefPubMedGoogle Scholar
  • 7 Santos N, Soares CC, Volotão EM, Albuquerque MC, Hoshino Y. Surveillance of rotavirus strains in Rio de Janeiro, Brazil, from 1997 to 1999. J Clin Microbiol 2003; 41 (07) 3399-3402
    CrossrefPubMedGoogle Scholar
  • 8 Parashar UD, Burton A, Lanata C. et al. Global mortality associated with rotavirus disease among children in 2004. J Infect Dis 2009; 200 (Suppl. 01) S9-S15
    CrossrefPubMedGoogle Scholar
  • 9 Tate JE, Burton AH, Boschi-Pinto C, Steele AD, Duque J, Parashar UD. WHO-coordinated Global Rotavirus Surveillance Network. 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis 2012; 12 (02) 136-141
    CrossrefPubMedGoogle Scholar
  • 10 GBD 2016 Causes of Death Collaborators. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet 2017; 390 (10100): 1151-1210
    CrossrefPubMedGoogle Scholar
  • 11 United States Agency for International Development, World Health Organization, and United Nations Children's Fund, Diarrhoea treatment guidelines including the new recommendations for the use of ORS and zinc supplementation for clinic-based healthcare workers. 2005 . Available at: www.mostproject.org/ZINC/Zinc_Updates_Apr05/Diarrhoea guidelines.pdf. Accessed September 17, 2020
    PubMed
  • 12 Tate JE, Burton AH, Bosch-Pinto C, Parashar UD. World Health Organization- Coordinated Global Rotavirus Surveillance Network. Global, regional, and national estimates of rotavirus mortality in children. Clin Infect Dis 2016; 62 (S2) S96-S105
    CrossrefPubMedGoogle Scholar
  • 13 Lang DR, Glass RI, Compans RW. Summary of the Fifth Rotavirus Vaccine Workshop. J Infect Dis 1996; 174 (Suppl. 01) S3-S4
    CrossrefPubMedGoogle Scholar
  • 14 Audu R, Omilabu SA, de Beer M, Peenze I, Steele AD. Diversity of human rotavirus VP6, VP7, and VP4 in Lagos State, Nigeria. J Health Popul Nutr 2002; 20 (01) 59-64
    PubMedGoogle Scholar
  • 15 Abiodun PO, Omoigberale A. Prevalence of nosocomial rotavirus infection in hospitalized children in Benin City, Nigeria. Ann Trop Paediatr 1994; 14 (01) 85-88
    CrossrefPubMedGoogle Scholar
  • 16 Iyoha O, Abiodun PO. Human rotavirus genotypes causing acute watery diarrhea among under-five children in Benin City, Nigeria. Niger J Clin Pract 2015; 18 (01) 48-51
    CrossrefPubMedGoogle Scholar
  • 17 Jinadu MK, Olusi SO, Agun JI, Fabiyi AK. Childhood diarrhoea in rural Nigeria. I. Studies on prevalence, mortality and socio-environmental factors. J Diarrhoeal Dis Res 1991; 9 (04) 323-327
    PubMedGoogle Scholar
  • 18 N.P.C. Population and Housing Census, National Population Commission (NPopC). 2006
    PubMed
  • 19 United Nations Children Fund, Levels and Trends in Child Mortality-Report 2010, Estimates Developed by the United Nations Inter-agency Group for Child Mortality Estimation, United Nations Children's Fund. 2010
  • 20 Ondo State Bureau of Statistics, Report of Integrated Household Survey in Ondo state 2011, Ondo State Bureau of Statistics Akure /NSDP/SRF/World Bank. 2012: 1-70
  • 21 World Health Organization, Generic protocol for (i) a hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children and (ii) a community-based survey on utilization of health care services for gastroenteritis in children, Geneva, Field test version. 2002 ; Document WHO/V&B/02.15
    PubMed
  • 22 Babalola MO, Odaibo GN, Olaleye OD, Alonge AO. Enteric adenovirus and norovirus gastroenteritis among under-5 years children in Owo, Ondo State, Nigeria. Br J Med Med Res 2015; 9 (08) 1-9
    CrossrefPubMedGoogle Scholar
  • 23 Troeger C, Forouzanfar M, Rao PC. et al. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect Dis 2017; 17 (09) 909-948
    CrossrefPubMedGoogle Scholar
  • 24 Troeger C, Khalil IA, Rao PC. et al. Rotavirus vaccination and the global burden of rotavirus diarrhea among children younger than 5 years. JAMA Pediatr 2018; 172 (10) 958-965
    CrossrefPubMedGoogle Scholar
  • 25 Adah MI, Rohwedder A, Olaleyle OD, Werchau H. Nigerian rotavirus serotype G8 could not be typed by PCR due to nucleotide mutation at the 3′ end of the primer binding site. Arch Virol 1997; 142 (09) 1881-1887
    CrossrefPubMedGoogle Scholar
  • 26 Adah MI, Wade A, Taniguchi K. Molecular epidemiology of rotaviruses in Nigeria: detection of unusual strains with G2P[6] and G8P[1] specificities. J Clin Microbiol 2001; 39 (11) 3969-3975
    CrossrefPubMedGoogle Scholar
  • 27 Aminu M, Esona MD, Geyer A, Steele AD. Epidemiology of rotavirus and astrovirus infections in children in northwestern Nigeria. Ann Afr Med 2008; 7 (04) 168-174
    CrossrefPubMedGoogle Scholar
  • 28 Asmah RH, Green J, Armah GE. et al. Rotavirus G and P genotypes in rural Ghana. J Clin Microbiol 2001; 39 (05) 1981-1984
    CrossrefPubMedGoogle Scholar
  • 29 Bonkoungou IJO, Sanou I, Bon F. et al. Epidemiology of rotavirus infection among young children with acute diarrhoea in Burkina Faso. BMC Pediatr 2010; 10: 94
    CrossrefPubMedGoogle Scholar
  • 30 Ayuk TB, Nyobe EC, Nchung JA. et al. Prevalence of diarrhoea and associated risk factors among children under-five years of age in Efoulan health district- Cameroon, sub- Saharan Africa. MOJ Public Health. 2018; 7 (06) 259-264
    CrossrefPubMedGoogle Scholar
  • 31 Jere KC, Sawyerr T, Seheri LM. et al. A first report on the characterization of rotavirus strains in Sierra Leone. J Med Virol 2011; 83 (03) 540-550
    CrossrefPubMedGoogle Scholar
  • 32 Armah GE, Pager CT, Asmah RH. et al. Prevalence of unusual human rotavirus strains in Ghanaian children. J Med Virol 2001; 63 (01) 67-71
    CrossrefPubMedGoogle Scholar
  • 33 Abebe A, Teka T, Kassa T. et al. Hospital-based surveillance for rotavirus gastroenteritis in children younger than 5 years of age in Ethiopia: 2007-2012. Pediatr Infect Dis J 2014; 33 (Suppl. 01) S28-S33
    CrossrefPubMedGoogle Scholar
  • 34 François PR, de Villiers FPR, Sawyerr TN, de Villiers GK. The incidence and clinical presentation of infantile rotavirus diarrhea in Sierra Leone. S Afr Med J 2009; 99: 4
    PubMedGoogle Scholar
  • 35 Kazemi A, Tabatabaie F, Agha-Ghazvini MR, Kelishadi R. The role of Rotavirusin acute Pediatric diarrhea in Isfahan, Iran. Pak J Med Sci 2006; 22 (03) 282-285
    PubMedGoogle Scholar
  • 36 Steele AD, Nimzing L, Peenze I. et al. Circulation of the novel G9 and G8 rotavirus strains in Nigeria in 1998/1999. J Med Virol 2002; 67 (04) 608-612
    CrossrefPubMedGoogle Scholar
  • 37 Bilcke J, Van Damme P, Van Ranst M, Hens N, Aerts M, Beutels P. Estimating the incidence of symptomatic rotavirus infections: a systematic review and meta-analysis. PLoS One 2009; 4 (06) e6060
    CrossrefPubMedGoogle Scholar
  • 38 Junaid SA, Umeh C, Olabode AO, Banda JM. Incidence of rotavirus infection in children with gastroenteritis attending Jos university teaching hospital, Nigeria. Virol J 2011; 8: 233
    CrossrefPubMedGoogle Scholar
  • 39 Parashar UD, Glass RI. Viral causes of gastroenteritis. In: Desselberger U, Gray J. ed. Viral Gastroenteritis. Vol. 9. Amsterdam: Elsevier; 2003: 9-21
    CrossrefGoogle Scholar
  • 40 Dennehy PH. Rotavirus vaccines: an overview. Clin Microbiol Rev 2008; 21 (01) 198-208
    CrossrefPubMedGoogle Scholar
  • 41 Gomwalk NE, Gosham LT, Umoh UJ. Rotavirus gastroenteritis in pediatric diarrhoea in Jos, Nigeria. J Trop Pediatr 1990; 36 (02) 52-55
    CrossrefPubMedGoogle Scholar
  • 42 Alam MM, Khurshid A, Shaukat S. et al. Epidemiology and genetic diversity of rotavirus strains in children with acute gastroenteritis in Lahore, Pakistan. PLoS One 2013; 8 (06) e67998
    CrossrefPubMedGoogle Scholar
  • 43 Agutu MT, Ongus J, Kombich J. et al. Prevalence and genetic diversity of rotavirus infection in children with acute gastroenteritis in a hospital setting, Nairobi Kenya in post vaccination era: a cross-sectional study. Pan Afr Med J 2017; 26: 38
    CrossrefPubMedGoogle Scholar
  • 44 Pennap G, Peenze I, De Beer M. Kwaga JKP. et al. VP6 subgroup and VP7 serotype of human rotavirus in Zaria, northern Nigeria. J Trop Pediatr 2000; 46 (06) 344-347
    CrossrefPubMedGoogle Scholar
  • 45 World Health Organization, Guidelines for Drinking-water Quality. 2011 . Available at: http://www.who.int/water_sanitation_health/publications/2011/dwq chapters/en/index.html. Accessed February 2, 2013
    PubMed
  • 46 Olusanya O, Taiwo O. Rotavirus as an aetiological agent of acute childhood diarrhoea in Ile-Ife, Nigeria. East Afr Med J 1989; 66 (02) 100-104
    PubMedGoogle Scholar
  • 47 Leite JPG, Alfieri AA, Woods PA, Glass RI, Gentsch JR. Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis. Arch Virol 1996; 141 (12) 2365-2374
    CrossrefPubMedGoogle Scholar
  • 48 Koroglu M, Yakupogullari Y, Otlu B. et al. A waterborne outbreak of epidemic diarrhea due to group A rotavirus in Malatya, Turkey. New Microbiol 2011; 34 (01) 17-24
    PubMedGoogle Scholar
  • 49 Omokhodion FO, Oyemade A, Sridhar MK, Olaseha IO, Olawuyi JF. Diarrhoea in children of Nigerian market women: prevalence, knowledge of causes, and management. J Diarrhoeal Dis Res 1998; 16 (03) 194-200
    PubMedGoogle Scholar
  • 50 Omatola CA, Olusola BA, Odaibo GN. Rotavirus infection among under five children presenting with gastroenteritis in Ibadan, Nigeria. Arch Basic and Appl Med 2016; 3: 3-8
    PubMedGoogle Scholar
  • 51 Nakawesi JS, Wobudeya E, Ndeezi G, Mworozi EA, Tumwine JK. Prevalence and factors associated with rotavirus infection among children admitted with acute diarrhea in Uganda. BMC Pediatr 2010; 10: 69
    CrossrefPubMedGoogle Scholar
  • 52 Binka FN, Anto FK, Oduro AR. Navrongo Rotavirus Research Group. et al. Incidence and risk factors of paediatric rotavirus diarrhoea in northern Ghana. Trop Med Int Health 2003; 8 (09) 840-846
    CrossrefPubMedGoogle Scholar
  • 53 Sénécal M, Brisson M, Lebel MH. MIRAGE study group. et al. Measuring the impact of rotavirus acute gastroenteritis episodes (MIRAGE): a prospective community-based study. Can J Infect Dis Med Microbiol 2008; 19 (06) 397-404
    CrossrefPubMedGoogle Scholar
  • 54 Aloun DS, Nyambat B, Phetsouvanh R. et al. Rotavirus diarrhoea among children aged less than 5 years at Mahosot Hospital, Vientiane, Lao PDR. Vaccine 2009; 27 (Suppl. 05) F85-F88
    CrossrefPubMedGoogle Scholar
  • 55 Gimenez-Sanchez F, Delgado-Rubio A, Martinon-Torres F, Bernaola-Iturbe E. Rotascore Research Group. Multicenter prospective study analysing the role of rotavirus on acute gastroenteritis in Spain. Acta Paediatr 2010; 99 (05) 738-742
    CrossrefPubMedGoogle Scholar
  • 56 Najafi A, Kargar M, Jafarpour T. Burden and typing of rotavirus group a in children with acute gastroenteritis in Shiraz, southern Iran. Iran Red Crescent Med J 2012; 14 (09) 531-540
    PubMedGoogle Scholar
  • 57 Uzoma EB, Chukwubuikem C, Omoyibo E, Tagbo O. Rota virus genotypes and the clinical severity of diarrhoea among children under 5 years of age. Niger Postgrad Med J 2016; 23 (01) 1-5
    CrossrefPubMedGoogle Scholar
  • 58 Rivest P, Proulx M, Lonergan G, Lebel MH, Bédard L. Hospitalisations for gastroenteritis: the role of rotavirus. Vaccine 2004; 22 (15-16): 2013-2017
    CrossrefPubMedGoogle Scholar
  • 59 Salinas B, Pérez Schael I, Linhares AC. et al. Evaluation of safety, immunogenicity and efficacy of an attenuated rotavirus vaccine, RIX4414: a randomized, placebo-controlled trial in Latin American infants. Pediatr Infect Dis J 2005; 24 (09) 807-816
    CrossrefPubMedGoogle Scholar
  • 60 Mast TC, DeMuro-Mercon C, Kelly CM, Floyd LE, Walter EB. The impact of rotavirus gastroenteritis on the family. BMC Pediatr 2009; 9 (11) 11
    CrossrefPubMedGoogle Scholar
  • 61 Zheng BJ, Chang RX, Ma GZ. et al. Rotavirus infection of the oropharynx and respiratory tract in young children. J Med Virol 1991; 34 (01) 29-37
    CrossrefPubMedGoogle Scholar
  • 62 Dodet B, Heseltine E, Saliou P. Rotaviruses in human and veterinary medicine. Trends Microbiol 1997; 5 (05) 176-178
    CrossrefPubMedGoogle Scholar
  • 63 Nitiema LW, Nordgren J, Ouermi D. et al. Burden of rotavirus and other enteropathogens among children with diarrhea in Burkina Faso. Int J Infect Dis 2011; 15 (09) e646-e652
    CrossrefPubMedGoogle Scholar
  • 64 Parashar UD, Gibson CJ, Bresee JS, Glass RI. Rotavirus and severe childhood diarrhea. Emerg Infect Dis 2006; 12 (02) 304-306
    CrossrefPubMedGoogle Scholar
  • 65 Waggie Z, Hawkridge A, Hussey GD. Review of rotavirus studies in Africa: 1976-2006. J Infect Dis 2010; 202: S23-S33
    CrossrefPubMedGoogle Scholar
  • 66 Odimayo MS, Olanrewaju WI, Omilabu SA, Adegboro B. Prevalence of rotavirus-induced diarrhea among children under 5 years in Ilorin, Nigeria. J Trop Pediatr 2008; 54 (05) 343-346
    CrossrefPubMedGoogle Scholar
  • 67 Ayolabi CI, Ojo DA, Armah GE. Electropherotypes and G-types of group A rotaviruses detected in children with diarrhea in Lagos, Nigeria. ISRN Virol 2013; DOI: 10.5402/2013/179871.
    CrossrefPubMedGoogle Scholar
  • 68 Alkali BR, Daneji AI, Magaji AA, Bilbis LS. Clinical symptoms of human rotavirus infection observed in children in Sokoto, Nigeria. Adv Virol 2015; 2015 DOI: 10.1155/2015/890957.
    CrossrefPubMedGoogle Scholar
  • 69 Oyejide CO, Fagbami AH. An epidemiological study of rotavirus diarrhoea in a cohort of Nigerian infants: II. Incidence of diarrhoea in the first two years of life. Int J Epidemiol 1988; 17 (04) 908-912
    CrossrefPubMedGoogle Scholar
  • 70 Centers for Disease Control and Prevention (CDC). Rotavirus surveillance--worldwide, 2001-2008. MMWR Morb Mortal Wkly Rep 2008; 57 (46) 1255-1257
    PubMedGoogle Scholar
  • 71 Enweronu-Laryea CC, Sagoe KW, Damanka S, Lartey B, Armah GE. Rotavirus genotypes associated with childhood severe acute diarrhoea in southern Ghana: a cross-sectional study. Virol J 2013; 10: 287
    CrossrefPubMedGoogle Scholar
  • 72 Ouédraogo N, Kaplon J, Bonkoungou IJO. et al. Prevalence and genetic diversity of enteric viruses in children with diarrhea in Ouagadougou, Burkina Faso. PLoS One 2016; 11 (04) e0153652
    CrossrefPubMedGoogle Scholar
  • 73 Glass RI, Kilgore PE, Holman RC. et al. The epidemiology of rotavirus diarrhea in the United States: surveillance and estimates of disease burden. J Infect Dis 1996; 174 (Suppl. 01) S5-S11
    CrossrefPubMedGoogle Scholar
  • 74 Satter SM, Gastanaduy PA, Islam K. et al. Hospital-based surveillance for rotavirus gastroenteritis among young children in Bangladesh: defining the potential impact of a rotavirus vaccine program. Pediatr Infect Dis J 2017; 36 (02) 168-172
    CrossrefPubMedGoogle Scholar