Cardiopulmonary bypass (CPB) is associated with platelet dysfunction (PD), an important cause of postoperative bleeding. The etiology of PD is not completely understood. We mapped the platelets' function during CPB to determine the etiology of PD. Platelets activation, measured by procaspase activating compound-1 and P-selectin expression (CD62P), after activation by adenosine diphosphate and thrombin receptor activator peptide, were decreased by protamine. Changes during CPB were insignificant. Platelet-leukocyte aggregation was increased by CPB but not by protamine. Platelet apoptosis marker, annexin V, was increased by protamine. Changes during CPB were insignificant. Our findings demonstrate that protamine given after CPB plays a central role in PD and count decrease.
2
Ranucci M,
Pistuddi V,
Di Dedda U,
Menicanti L,
De Vincentiis C,
Baryshnikova E.
Platelet function after cardiac surgery and its association with severe postoperative bleeding: the PLATFORM study. Platelets 2019; 30 (07) 908-914
4
Adamik B,
Kübler A,
Gozdzik A,
Gozdzik W.
Prolonged cardiopulmonary bypass is a risk factor for intestinal ischaemic damage and endotoxaemia. Heart Lung Circ 2017; 26 (07) 717-723
5
Chalmers J,
Pullan M,
Mediratta N,
Poullis M.
A need for speed? Bypass time and outcomes after isolated aortic valve replacement surgery. Interact Cardiovasc Thorac Surg 2014; 19 (01) 21-26
7
Vinholt PJ,
Hvas AM,
Nybo M.
An overview of platelet indices and methods for evaluating platelet function in thrombocytopenic patients. Eur J Haematol 2014; 92 (05) 367-376
10
Olsson A,
Alfredsson J,
Thelander M,
Svedjeholm R,
Berglund JS,
Berg S.
Activated platelet aggregation is transiently impaired also by a reduced dose of protamine. Scand Cardiovasc J 2019; 53 (06) 355-360
