Abstract
Objective To present an innovative device that applies the double centrifugation method to
obtain platelet-rich plasma (PRP), assessing whether there was an effective increase
in the concentration of platelets.
Method Ten volunteers underwent blood collection. The samples were separated in 20 ml syringes,
sealed and subjected to the double centrifugation protocol at 1,100 revolutions per
minute (rpm) for 15 minutes, resulting in the separation of red blood cells, plasma
with platelets, and leukocytes. Then, 10 ml syringes were added to remove 9 ml, respecting
the “buffy coat” parameter, collecting 8 ml above and 1 ml below for the second centrifugation
and transferring again to the 20 ml syringe. The plasma was again centrifuged at 1,550 rpm
for 10 minutes; as a result, it was divided into two parts: at the top, consisting
of low platelet plasma (LPP), and at the bottom, by the platelet button. Part of the
LPP was discarded, leaving only 3 ml with the platelet button. The cells were then
counted.
Results This innovative device was able to increase the concentration of platelets by almost
three times compared with the baseline. In addition, the preparation time for the
PRP was adequate, lasting only 35 to 40 minutes.
Conclusions Platelet-rich plasma was successfully obtained by the double centrifuge protocol,
allowing its clinical use. In addition, obtaining through the presented device promotes
greater applicability in the preparation of PRP in specific centers, furthermore,
being a quick and economical way to obtain PRP.
Keywords
device - platelet - platelet-rich plasma
