Download PDF
CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2022; 26(01): e119-e124
DOI: 10.1055/s-0040-1722161
Original Research

Antioxidant Effect of Curcumin on the Prevention of Oxidative Damage to the Cochlea in an Ototoxic Rat Model Based on Malondialdehyde Expression

Tengku Siti Hajar Haryuna 
1   Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
,
Dyah Fauziah 
2   Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
,
Sari Anggraini 
1   Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
,
M Pahala Hanafi Harahap 
1   Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
,
Juliandi Harahap 
3   Department of Community Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia
› Author Affiliations
› Further Information
Also available at
   Permissions and Reprints

Abstract

Introduction Aminoglycoside, as an antimicrobial medication, also has side-effects on the inner ears, bringing about hearing disorders. Curcumin has been proven to be a strong scavenger against various reactive oxygen species (ROS), and the increase in ROS production is considered to play an important role in the process of hearing disorder.

Objective To prove that curcumin is an effective antioxidant to prevent cochlear damage based on malondialdehyde (MDA) expression.

Methods The present research used 32 Rattus norvegicus, of the Wistar lineage, randomly divided into 8 groups: negative control, ototoxic control (a single dose of 40 mg/ml of gentamicin via intratympanic injection), 2 groups submitted to ototoxic control + curcumin treatment (100 mg/kg, 200 mg/kg), 2 groups who iunderwent ototoxic control + curcumin treatment for 7 days, and two groups submitted to curcumin treatment as prevention for 3 days + ototoxic induction.

Results The results showed that the lowest dosage of curcumin (100 mg/kg) could decrease MDA expression on the cochlear fibroblastic wall of the ototoxic model; however using greater doses of curcumin (200 mg/kg) for 7 days would provide a better effect. Curcumin could also significantly decrease MDA expression when it was administered during the preototoxic exposure.

Conclusion Curcumin can be used as a therapy for ototoxic prevention based on the decrease in MDA expression.

Keywords

curcumin - malondialdehyde - cochlear fibroblast - reactive oxygen species - ototoxicity - gentamicin


Publication History

Received: 19 January 2020

Accepted: 22 October 2020

Article published online:
09 August 2021

© 2021. Fundação Otorrinolaringologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Thieme Revinter Publicações Ltda.
Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil

 

  • References

  • 1 Rogers C, Petersen L. Aminoglycoside-induced balance deficits: a review of vestibulotoxicity. S Afr Fam Pract 2011; 53 (05) 419-424
    CrossrefSearch in Google Scholar
  • 2 ASHA, Evidence-Based Systematic Review (EBSR): Drug-Induced Hearing Loss- - Amynoglycosides, American Speech-Language-Hearing Association. (Accessed March 12 th, 2019) at: http:/www.asha.org/uplodedFiles/EBSRAminoglycosides.pdf
  • 3 Huth ME, Ricci AJ, Cheng AG. Mechanisms of aminoglycoside ototoxicity and targets of hair cell protection. Int J Otolaryngol 2011; 2011: 937861
    PubMedSearch in Google Scholar
  • 4 Schacht J, Talaska AE, Rybak LP. Cisplatin and aminoglycoside antibiotics: hearing loss and its prevention. Anat Rec (Hoboken) 2012; 295 (11) 1837-1850
    CrossrefPubMedSearch in Google Scholar
  • 5 Ciuman RR. Inner ear symptoms and disease: pathophysiological understanding and therapeutic options. Med Sci Monit 2013; 19: 1195-1210
    CrossrefPubMedSearch in Google Scholar
  • 6 Xie J, Talaska AE, Schacht J. New developments in aminoglycoside therapy and ototoxicity. Hear Res 2011; 281 (1-2): 28-37
    CrossrefPubMedSearch in Google Scholar
  • 7 Lin CD, Kao MC, Tsai MH. et al. Transient ischemia/hypoxia enhances gentamicin ototoxicity via caspase-dependent cell death pathway. Lab Invest 2011; 91 (07) 1092-1106
    CrossrefPubMedSearch in Google Scholar
  • 8 Chirtes F, Albu S. Prevention and restoration of hearing loss associated with the use of cisplatin. BioMed Res Int 2014; 2014: 925485
    CrossrefPubMedSearch in Google Scholar
  • 9 Ho E, Karimi Galougahi K, Liu CC, Bhindi R, Figtree GA. Biological markers of oxidative stress: Applications to cardiovascular research and practice. Redox Biol 2013; 1: 483-491
    CrossrefPubMedSearch in Google Scholar
  • 10 Moghimian M, Abtahi-Evari SH, Shokoohi M. et al. Effect of Syzygium aromaticum (clove) extract on seminiferous tubules and oxidative stress after testicular torsion in adult rats. Physiol Pharmacol 2017; 21: 343-350
    Search in Google Scholar
  • 11 Moghimian M, Soltani M, Abtahi H, Shokoohi M. Effect of vitamin C on tissue damage and oxidative stress following tunica vaginalis flap coverage after testicular torsion. J Pediatr Surg 2017; 52 (10) 1651-1655
    CrossrefPubMedSearch in Google Scholar
  • 12 Marrocco I, Altieri F, Peluso I. Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans. Oxid Med Cell Longev 2017; 2017: 6501046
    CrossrefPubMedSearch in Google Scholar
  • 13 Soltani M, Moghimian M, Abtahi-Eivari SH, Shoorei H, Khaki A, Shokoohi M. Protective Effects of Matricaria chamomilla Extract on Torsion/ Detorsion-Induced Tissue Damage and Oxidative Stress in Adult Rat Testis. Int J Fertil Steril 2018; 12 (03) 242-248
    PubMedSearch in Google Scholar
  • 14 Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014; 2014: 360438
    CrossrefPubMedSearch in Google Scholar
  • 15 Bertazza L, Barollo S, Mari ME. et al. Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines. Molecules 2019; 24 (12) 1-12
    Search in Google Scholar
  • 16 Girdhani S, Ahmed MM, Mishra KP. Enhancement of Gamma Radiation-induced Cytotoxicity of Breast Cancer Cells by Curcumin. Mol Cell Pharmacol 2009; 1: 208-217
    Search in Google Scholar
  • 17 Yeung AWK, Horbańczuk M, Tzvetkov NT. et al. Curcumin: Total-Scale Analysis of the Scientific Literature. Molecules 2019; 24 (07) 1-14
    Search in Google Scholar
  • 18 Elseweidy MM, Younis NN, Elswefy SE. et al. Atheroprotective potentials of curcuminoids against ginger extract in hypercholesterolaemic rabbits. Nat Prod Res 2015; 29 (10) 961-965
    CrossrefPubMedSearch in Google Scholar
  • 19 Hewlings SJ, Kalman DS. Curcumin: A Review of Its Effects on Human Health. Foods 2017; 6 (10) 1-11
    CrossrefPubMedSearch in Google Scholar
  • 20 Sagit M, Somdas MA, Korkmaz F, Akcadag A. The ototoxic effect of intratympanic terbinafine applied in the middle ear of rats. J Otolaryngol Head Neck Surg 2013; 42: 13
    CrossrefPubMedSearch in Google Scholar
  • 21 Suzuki M, Ushio M, Yamasoba T. Time course of apoptotic cell death in guinea pig cochlea following intratympanic gentamicin application. Acta Otolaryngol 2008; 128 (07) 724-731
    CrossrefPubMedSearch in Google Scholar
  • 22 Toydemir T, Kanter M, Erboga M. et al. Antioxidative, antiapoptotic, and proliverative effect of curcumin on liver regeneration after partial hepatectomy in rats. Toxicol Ind Health 2012; •••: 1-11
    Search in Google Scholar
  • 23 Walvekar MV, Potphode ND, Desai SS. et al. Histological Studies on Islets of Langerhans of Pancreas in Diabetic Mice after Curcumin Administration. IJPCR 2016; 8: 1314-1318
    Search in Google Scholar
  • 24 Fedchenko N, Reifenrath J. Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review. Diagn Pathol 2014; 9: 221
    CrossrefPubMedSearch in Google Scholar
  • 25 Ellenbroek B, Youn J. Rodent models in neuroscience research: is it a rat race?. Dis Model Mech 2016; 9 (10) 1079-1087
    CrossrefPubMedSearch in Google Scholar
  • 26 Dierckx N, Horvath G, van Gils C. et al. Oxidative stress status in patients with diabetes mellitus: relationship to diet. Eur J Clin Nutr 2003; 57 (08) 999-1008
    CrossrefPubMedSearch in Google Scholar
  • 27 Poirrier AL, Pincemail J, Van Den Ackerveken P, Lefebvre PP, Malgrange B. Oxidative stress in the cochlea: an update. Curr Med Chem 2010; 17 (30) 3591-3604
    CrossrefPubMedSearch in Google Scholar
  • 28 Shokoohi M, Shoorei H, Soltani M, Abtahi-Eivari SH, Salimnejad R, Moghimian M. Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats. Andrologia 2018; 50 (07) e13047
    CrossrefPubMedSearch in Google Scholar
  • 29 Shookoohi M, Madarek EOS, Khaki A. et al. Investigating the Effects of Onion Juice on Male Fertility Factors and Pregnancy Rate After Testicular Torsion/ Detorsion by Intrauterine Insemination Methode. Int J Women's Health Reprod Sci 2018; 6 (04) 499-505
    CrossrefSearch in Google Scholar
  • 30 Deavall DG, Martin EA, Horner JM, Roberts R. Drug-induced oxidative stress and toxicity. J Toxicol 2012; 2012: 645460
    CrossrefPubMedSearch in Google Scholar
  • 31 Üstün Bezgin S, Uygur KK, Gökdoğan Ç, Elmas Ç, Göktaş G. The Effects of Riluzole on Cisplatin-induced Ototoxicity. Int Arch Otorhinolaryngol 2019; 23 (03) e267-e275
    Thieme ConnectPubMedSearch in Google Scholar
  • 32 Nagamani M, Prahaladu P, Vijayababu VPSS. et al. Lipid Peroxidation Product As A Marker Of Oxidative Stress In Psoriasis -A Case Control Study In North Coastal Andhra Pradesh. IOSR-JDMS 2015; 14: 18-20
    Search in Google Scholar
  • 33 Molina-Jijón E, Tapia E, Zazueta C. et al. Curcumin prevents Cr(VI)-induced renal oxidant damage by a mitochondrial pathway. Free Radic Biol Med 2011; 51 (08) 1543-1557
    CrossrefPubMedSearch in Google Scholar
  • 34 Correa F, Buelna-Chontal M, Hernández-Reséndiz S. et al. Curcumin maintains cardiac and mitochondrial function in chronic kidney disease. Free Radic Biol Med 2013; 61: 119-129
    CrossrefPubMedSearch in Google Scholar
  • 35 Kuhad A, Pilkhwal S, Sharma S, Tirkey N, Chopra K. Effect of curcumin on inflammation and oxidative stress in cisplatin-induced experimental nephrotoxicity. J Agric Food Chem 2007; 55 (25) 10150-10155
    CrossrefPubMedSearch in Google Scholar
  • 36 Alrawaiq NS, Abdullah A. A Review of Antioxidant Polyphenol Curcumin and its Role in Detoxification. Int J Pharm Tech Res 2014; 6: 280-289
    Search in Google Scholar
  • 37 Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 2005; 15 (04) 316-328
    CrossrefPubMedSearch in Google Scholar