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DOI: 10.1055/s-0041-109771
Was soll der Chirurg vom histopathologischen Präparate-Processing wissen?
What Surgeons Need to Know About the Histopathological Processing of a SpecimenPublikationsverlauf
Publikationsdatum:
02. Mai 2016 (online)
Zusammenfassung
Zahlreiche Einflussfaktoren beeinflussen den Zustand des Gewebes auf dem Weg von der Entnahme aus dem Körper bis unter das Mikroskop des Pathologen. Diese Faktoren lassen sich unterteilen in solche, die der Chirurg beeinflussen kann, und solche, die sich in der Pathologie abspielen, deren mögliche Fehlerquellen und Einflüsse auf die Diagnose er kennen sollte. Die Faktoren können die Diagnosen an konventionellen Färbungen verändern, besonders aber die Diagnosen an immunhistochemisch oder molekularpathologisch untersuchten Geweben. Deswegen ist eine Standardisierung der Gewebebearbeitung zu fordern. Diese ist extrem wichtig für Biobankproben, insbesondere für Biobankgewebeproben, für die ein Protokoll der Gewebebehandlung zu fordern ist.
Abstract
A multitude of factors influence the state of tissue samples on their way from the biopsy site in the body to the pathologistʼs microscope. Some of these factors can be influenced by surgeons, while others are dealt with in pathology departments, but surgeons should know potential pitfalls and caveats and their influence on the pathohistological diagnosis. These factors influence diagnoses made on conventional stains, but even more so the results of immunohistochemical stains and molecular pathology examinations. Therefore, the work-up of tissue samples should be standardised. This is of utmost importance for biobank tissue samples, especially those for which a tissue treatment protocol is recommended.
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