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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(03): 546-550
DOI: 10.1055/s-0041-1723067
Original Article

Bactericidal Effect of a Novel Alkaline EDTA Root Canal Cleaning Solution

Takenori Sato
1   Department of Oral Science, Division of Oral Biochemistry, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Ryuji Fujimaki
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Jiro Suzuki
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Nobushiro Hamada
3   Department of Oral Science, Division of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Nobuyuki Tani-Ishii
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Keisuke Handa
1   Department of Oral Science, Division of Oral Biochemistry, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
› Author Affiliations Funding This work was supported by JSPS KAKENHI under grant numbers JP16K11568, and JP18K09592.
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Abstract

Objectives In this study, we aimed to evaluate the bactericidal effect and cytotoxicity of an ethylenediaminetetra-acetic acid (EDTA)-based root canal irrigant solution capable of efficiently removing both the organic matter and the smear layer. We prepared a strong alkaline EDTA (AE) solution with an acid buffer capacity similar to that of sodium hypochlorite.

Materials and Methods AE was used at concentrations of 1%, 2%, and 3%. The bactericidal effect of AE on Enterococcus faecalis was evaluated by determining the colony number and biofilm removal rate. Biofilms were visualized using a Live/Dead BacLight bacterial viability kit. Viability of AE-treated cells were determined using a CCK-8 cell counting assay.

Statistical Analysis One-way analysis of variance followed by a Dunnett’s multiple comparison test were used for comparisons among groups.

Results Significant reduction in cell viability and biofilm formation were observed in case of 3% and 2% AE. AE exerted bactericidal effects in a concentration-dependent manner. Damage of normal human fibroblasts was not observed at any of the AE concentrations.

Conclusions Our results suggest that the AE solution could be used as an effective canal irrigant for the removal of bacterial biofilm.

Keywords

ethylenediaminetetraacetic acid - sodium hypochlorite - biofilm - root canal irrigant


Publication History

Article published online:
10 March 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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