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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(02): 314-320
DOI: 10.1055/s-0041-1724076
Artigo Original
Joelho

BMP-4, TGF-β and Smad3 as Modulators of Synovial Fluid Cells Viability[*]

Article in several languages: português | English
Eduardo Branco de Sousa 
1   Divisão de Ensino e Pesquisa, Instituto Nacional de Ortopedia e Traumatologia Jamil Haddad, Rio de Janeiro, RJ, Brasil
,
Vivaldo Moura Neto 
2   Laboratório de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Rio de Janeiro, RJ, Brasil
,
Diego Pinheiro Aguiar 
3   Laboratório de Biomodelos e Protótipos, Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brasil
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Abstract

Objective Our goal was to evaluate the modulation of the synovial fluid cells (SFC) from patients with and without osteoarthritis (OA) by bone morphogenetic protein 4 (BMP-4), Smad-3 and transforming growth factor beta (TGF-β).

Methods Synovial fluid was collected from patients submitted to knee arthroscopy or replacement and were centrifuged to isolate cells from the fluid. Cells were cultured for 21 days and characterized as mesenchymal stem cells (MSCs) according to the criteria of the International Society of Cell Therapy. Then, we performed an [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (MTT) assay after exposing cells with and without OA to TGF-β, Smad3 and BMP-4 pathway inhibitors and to different concentrations of BMP4.

Results Exposure to the TGF-β, Smad3 and BMP-4 inhibitors modifies the mitochondrial activity of the SFCs. The activity of the SFCs is modified by influences of increasing concentrations of BMP4, but there is no difference in cellular activity between patients with and without OA.

Conclusion TGF-β, Smad3 and BMP-4 modulate the activity of SFCs from patients with and without knee OA.

Keywords

mesenchymal stem cells - osteoarthritis - synovial fluid cells - TGF-β pathway

Author Contributions

de Souza E. B.: Concept and design, provision of study material or patients, collection and assembly of data, data analysis and interpretation, manuscript writing, final approval of the manuscript.


Moura Neto V.: Data analysis and interpretation, final approval of the manuscript.


Aguiar D. P.: Concept and design, collection and assembly of data, data analysis and interpretation, manuscript writing, final approval of the manuscript.


All authors read and approved the final manuscript.


* Work developed at the Divisão de Ensino e Pesquisa, Instituto Nacional de Ortopedia e Traumatologia Jamil Haddad, Rio de Janeiro, RJ, Brazil.




Publication History

Received: 31 May 2020

Accepted: 16 September 2020

Article published online:
31 March 2021

© 2021. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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