Effectiveness of Gastric Electrical Stimulation is Related with Regional Expression Of Interstitial Cells of Cajal in Porcine Stomach

Aims Stomach is composed with several types of interstitial cells of Cajal (ICC). Among ICCs, myenteric ICC (ICC-MY) serve as a pacemaker which creates the bioelelctrical slow wave potential that leads to contraction of the smooth muscle. Moreover, the ICC-MY responses to an external electrical stimulation. We performed to investigate a proper electrical parameter to cause resonance of contraction of muscle.

Methods We conducted a square wave-electrical stimulation with variable duty cycle of 1000, 1500 and 2000 us, fixing frequency of 100 Hz, potential of 5 V, and delay of 100 ms for rat. We assessed immunohistochemistry of c-kit which are necessary for the generation of slow waves. C-kit positive ICC for body and fundus part of the rat’ stomach was analyzed by measuring changes in area with ImageJ.

Results The area of c-kit positive ICC-MY for the body and fundus part of stomach was linearly decreased with increasing in duty cycle, that is, stimulation strength. The decreasing rate was the same for the body and fundus. The decreasing means ICC-MY act as responder to external electric stimulation. This stimulation may be utilized to activate the stomach forcely.

Conclusions Intensity and distribution of interstitial cells of cajal in gastrointestinal tract might affect the efficacy of gastric electrical stimulation.

Citation: Kim SH, Kim HB, Chun HJ et al. eP171 EFFECTIVENESS OF GASTRIC ELECTRICAL STIMULATION IS RELATED WITH REGIONAL EXPRESSION OF INTERSTITIAL CELLS OF CAJAL IN PORCINE STOMACH. Endoscopy 2021; 53: S152.

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Artikel online veröffentlicht:
19. März 2021