Open Access
CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S124
DOI: 10.1055/s-0041-1727968
Abstracts
Head-Neck-Oncology: HPV / Tumor Marker

Tumor-exclusive peptides from shared and individual antigens in the HLA-ligandome of oropharyngeal squamous cell carcinoma

Authors

  • S Laban

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • J Ezic

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • L Bichmann

    2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen

  • D Engelhardt

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • HA. Kestler

    4   Universität Ulm, Institut für Medizinische Systembiologie, Ulm

  • A von Witzleben

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • J Thomas

    5   University of Southampton, Faculty of Medicine, Cancer Sciences Unit, Southampton, United Kingdom

  • J Mytilineos

    6   Deutsches Rotes Kreuz Blutspendedienst, Institut für Klinische Transfusionsmedizin und Immungenetik, Ulm

  • D Fürst

    6   Deutsches Rotes Kreuz Blutspendedienst, Institut für Klinische Transfusionsmedizin und Immungenetik, Ulm

  • J Döscher

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • PJ. Schuler

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • C Brunner

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • TK. Hoffmann

    1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm

  • H-G Rammensee

    2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen

  • S Stevanovic

    2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen

  • L Mühlenbruch

    2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen
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Introduction Cancer-antigens are presented to the immune system via Human leucocyte antigens (HLA). The HLA-ligandome in oropharyngeal squamous cell carcinoma (OPSCC) has not been described previously.

Material and Methods OPSCC tumor biopsies from 40 patients and tonsillar tissue from 5 healthy donors were analyzed. HLA molecules were extracted from fresh frozen OPSCC biopsies, HLA ligands were isolated by high performance liquid chromatography (HPLC) and analyzed by tandem mass spectrometry (MS/MS). Tumor-exclusive peptides (TEP) were identified by comparative profiling against normal tonsil tissue and an in-house benign and malignant HLA-ligandome database. Whole exome sequencing of RNA and DNA was also performed.

Results OPSCC were classified as HPV+ (n=22) or HPV- (n=18) based on RNA-seq data. In total, 25228 HLA class I presented peptides from 9485 source proteins and 15203 HLA class II presented peptides from 4634 source proteins were detected. Peptides derived from the cancer-testis antigens IMP3, KDM5B, CTNNA2 and ATAD2 were found in = 30 %  of patients. In total, 3746 HLA class I TEP and 5096 HLA class II TEP were identified. Some TEP were only found in HPV+ or HPV-, whereas others were shared among both, HPV+ and HPV-.

Conclusion The HLA-ligandome of OPSCC contains both shared and individual TEP and differs by HPV-status. Based on these results, semi-personalized or personalized cancer vaccines could be developed.

Poster-PDF A-1495.pdf

DFG (GRK-2254), Universität Ulm (Clinician Scientist Programme)



Publication History

Article published online:
13 May 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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