CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S311-S312
DOI: 10.1055/s-0041-1728941
Abstracts
Tissue Engineering / Stem Cells

Electrospun Biomimetic SF/PCL Tympanic Membrane Implant: Experimental Study of Material Design, Acousto-Mechanical Properties and Biocompatability

Z Chen
1   Technische Universität Dresden, Carl Gustav Carus Faculty of Medicine, Department of Otorhinolaryngology, Head and Neck Surgery, Ear Research Center Dresden, Dresden
,
L Benecke
2   Technische Universität Dresden, Faculty of Mechanical Science and Engineering, Institute of Textile Machinery and High Performance Material Technology, Dresden
,
I Zeidler-Rentzsch
1   Technische Universität Dresden, Carl Gustav Carus Faculty of Medicine, Department of Otorhinolaryngology, Head and Neck Surgery, Ear Research Center Dresden, Dresden
,
M von Witzleben
3   Technische Universität Dresden, Carl Gustav Carus Dresden Faculty of Medicine, Center for Translational Bone, Joint and Soft Tissue Research, Dresden
,
M Bornitz
1   Technische Universität Dresden, Carl Gustav Carus Faculty of Medicine, Department of Otorhinolaryngology, Head and Neck Surgery, Ear Research Center Dresden, Dresden
,
D Aibibu
2   Technische Universität Dresden, Faculty of Mechanical Science and Engineering, Institute of Textile Machinery and High Performance Material Technology, Dresden
,
C Cherif
2   Technische Universität Dresden, Faculty of Mechanical Science and Engineering, Institute of Textile Machinery and High Performance Material Technology, Dresden
,
T Zahnert
1   Technische Universität Dresden, Carl Gustav Carus Faculty of Medicine, Department of Otorhinolaryngology, Head and Neck Surgery, Ear Research Center Dresden, Dresden
,
M Neudert
1   Technische Universität Dresden, Carl Gustav Carus Faculty of Medicine, Department of Otorhinolaryngology, Head and Neck Surgery, Ear Research Center Dresden, Dresden
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Content

Introduction Electrospun fiber membranes made of silk-fibroin (SF) and synthetic poly(caprolactone) (PCL) have a high potential to mimetic natural collagen fiber morphologies of the tympanic membrane (TM). In this work, experimental investigations on material design, acousto-mechanical properties and biocompatibility were performed to develop a biomimetic SF/PCL TM implant based on our previous simulation results.

Methods Material design was evaluated by varying electrospinning solution (different viscosity and amount of SF) and varying technical parameters (voltage, spinning distance and spinning time). The acoustic vibration behavior of scaffolds was determined by laser Doppler vibrometer (LDV) in the frequency range between 0.1 and 5 kHz. Uniaxial compression/strain tests were performed employing an indenter to evaluate the mechanical properties. Biocompatibility was investigated by cultivating middle ear epithelium cells on the scaffolds and determining vitality and cytotoxicity at different time points.

Results According to the material design, the fiber properties, fiber diameter, porosity and thickness of the scaffolds varied. This led to differences in hydrophilic properties, acousto-mechanical behavior and biocompatibility. The silk-fibroin contributed to a better hydrophilic property and biocompatibility. Scaffolds with ratio of SF to PCL 1:2 showed the closest acoustic vibration behavior to human TM.

Conclusion Electrospinning is able to produce desirable TM implant with properties comparable to normal eardrum. The scaffolds with the best compromise between acousto-mechanical properties and biocompatibility will be selected and transferred to the subsequent in vitro and in vivo clinical studies.

Poster-PDF A-1479.pdf



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Artikel online veröffentlicht:
13. Mai 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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